| Construct . | IK/INa (−100mV) . | ILi/INa (−100mV) . | ICs/INa (−100 mV) . |
|---|---|---|---|
| WT hENaC | −0.04 ± 0.04 | 1.2 ± 0.2 | −0.04 ± 0.04 |
| αβ(T35V)γ | 0.02 ± 0.02a | 1.6 ± 0.3 | 0.01 ± 0.03 |
| αβγ(T38V) | n.d. | 1.6 ± 0.8 | n.d. |
| αβ(T35V)γ(T38V) | 0.04 ± 0.04b | 0.8 ± 0.2 | −0.00 ± 0.00 |
| α(T67V)β(N34A)γ | n.d. | 1.6 ± 0.2 | n.d. |
| α(T67V)βγ | 0.03 ± 0.06c | 1.8 ± 0.7 | −0.01 ± 0.02 |
| α(T66V)β(T33V) γ | −0.00 ± 0.01 | 1.3 ± 0.3 | −0.01 ± 0.02 |
| α(T66V)βγ | 0.03 ± 0.02c | 1.5 ± 0.1 | −0.04 ± 0.06 |
| α(N65A)β(N32A)γ(N35A) | −0.00 ± 0.01 | 2.3 ± 0.8a | 0.00 ± 0.03 |
| α(N65A)βγ | 0.02 ± 0.01 | 1.6 ± 0.4 | −0.06 ± 0.11 |
| α(N64A)β(D31A)γ(L34A) | 0.02 ± 0.03a | 1.4 ± 0.2 | 0.01 ± 0.03 |
| α(N64A)βγ | 0.02 ± 0.01 | 1.6 ± 0.4 | −0.06 ± 0.11 |
| α(C63A)β(C30A)γ(C33A) | 0.00 ± 0.01 | 2.0 ± 0.7 | −0.01 ± 0.01 |
| α(C63A)βγ | −0.02 ± 0.02 | 1.7 ± 0.6 | −0.01 ± 0.02 |
| α(F62A)β(Y29A)γ | 0.01 ± 0.02 | 3.7 ± 2.1d | 0.01 ± 0.03 |
| α(F62A)βγ | 0.01 ± 0.01a | 1.3 ± 0.5 | 0.00 ± 0.01 |
| Construct . | IK/INa (−100mV) . | ILi/INa (−100mV) . | ICs/INa (−100 mV) . |
|---|---|---|---|
| WT hENaC | −0.04 ± 0.04 | 1.2 ± 0.2 | −0.04 ± 0.04 |
| αβ(T35V)γ | 0.02 ± 0.02a | 1.6 ± 0.3 | 0.01 ± 0.03 |
| αβγ(T38V) | n.d. | 1.6 ± 0.8 | n.d. |
| αβ(T35V)γ(T38V) | 0.04 ± 0.04b | 0.8 ± 0.2 | −0.00 ± 0.00 |
| α(T67V)β(N34A)γ | n.d. | 1.6 ± 0.2 | n.d. |
| α(T67V)βγ | 0.03 ± 0.06c | 1.8 ± 0.7 | −0.01 ± 0.02 |
| α(T66V)β(T33V) γ | −0.00 ± 0.01 | 1.3 ± 0.3 | −0.01 ± 0.02 |
| α(T66V)βγ | 0.03 ± 0.02c | 1.5 ± 0.1 | −0.04 ± 0.06 |
| α(N65A)β(N32A)γ(N35A) | −0.00 ± 0.01 | 2.3 ± 0.8a | 0.00 ± 0.03 |
| α(N65A)βγ | 0.02 ± 0.01 | 1.6 ± 0.4 | −0.06 ± 0.11 |
| α(N64A)β(D31A)γ(L34A) | 0.02 ± 0.03a | 1.4 ± 0.2 | 0.01 ± 0.03 |
| α(N64A)βγ | 0.02 ± 0.01 | 1.6 ± 0.4 | −0.06 ± 0.11 |
| α(C63A)β(C30A)γ(C33A) | 0.00 ± 0.01 | 2.0 ± 0.7 | −0.01 ± 0.01 |
| α(C63A)βγ | −0.02 ± 0.02 | 1.7 ± 0.6 | −0.01 ± 0.02 |
| α(F62A)β(Y29A)γ | 0.01 ± 0.02 | 3.7 ± 2.1d | 0.01 ± 0.03 |
| α(F62A)βγ | 0.01 ± 0.01a | 1.3 ± 0.5 | 0.00 ± 0.01 |
Relative amiloride-sensitive currents determined for WT hENaC and hENaC mutants containing the designated amino acid substitutions. Data are shown as mean ± SD, and n refers to the number of individual oocytes; n.d., not determined. Values for mutants were compared with WT hENaC using one-way ANOVA with Dunnett’s multiple comparisons test; n = 3–7.
P < 0.05.
P < 0.001.
P < 0.01.
P < 0.0001.