Table 6. Relative ion permeabilities and... | Rockefeller University Press

Table 6.

Relative ion permeabilities and pH₅₀ values of mASIC1a mutants

Construct	P_Na+/P_K+	P_Na+/P_Li+	P_Cs+/P_Na+	pH₅₀
WT	8.0 ± 1.6	1.1 ± 0.2	29.9 ± 17.2	6.8 ± 0.03
F21A	1.0 ± 0.1^a	1.2 ± 0.2	0.9 ± 0.1^b	7.0 ± 0.04^a
F21L	1.0 ± 0.1^a	1.0 ± 0.1	1.4 ± 0.3^b	7.1 ± 0.05^c
F21W	4.1 ± 1.5^a	1.0 ± 0.3	10.4 ± 7.0^b	7.1 ± 0.08^a
A22G	1.1 ± 0.1^a	1.1 ± 0.1	1.1 ± 0.1^b	7.1 ± 0.02^a
A22L	1.0 ± 0.1^a	1.3 ± 0.3	0.9 ± 0.0^c	7.1 ± 0.01^a
S23A	9.0 ± 2.0	1.3 ± 0.2	40.4 ± 22.9	6.6 ± 0.01
S23E	8.3 ± 0.8	1.5 ± 0.3	46.2 ± 32.4	6.8 ± 0.01
S24A	9.8 ± 1.1	1.1 ± 0.1	44.2 ± 15.1	6.8 ± 0.1
S24E	10.7 ± 3.9^b	n.d.	40.3 ± 21.5	6.8 ± 0.02
S25A	5.6 ± 1.6^b	1.1 ± 0.2	16.6 ± 4.7	6.8 ± 0.07
S25E	1.5 ± 0.6^a	1.5 ± 0.5	2.0 ± 1.3^a	7.1 ± 0.1^a
T26C	0.9 ± 0.2^a	1.0 ± 0.3	1.0 ± 0.2^a	7.1 ± 0.03^a
T26E	1.1 ± 0.3^a	1.3 ± 0.5	1.1 ± 0.6^a	7.2 ± 0.05^a
T26V	0.9 ± 0.3^a	1.0 ± 0.5	1.1 ± 0.2^a	7.2 ± 0.04^a
T26S	5.7 ± 1.8^b	1.2 ± 0.4	9.7 ± 1.7^d	6.8 ± 0.04
H28A	0.8 ± 0.1^a	1.6 ± 0.3	1.1 ± 0.1^c	7.3 ± 0.06^a
G29A	1.1 ± 0.2^a	1.5 ± 0.6	1.6 ± 0.5 ^c	7.2 ± 0.04^a
I33A	1.0 ± 0.2^a	1.0 ± 0.4	1.1 ± 0.1 ^c	7.0 ± 0.03^a
K42Q	3.7 ± 0.3^a	0.7 ± 0.2	3.6 ± 1.7 ^c	6.8 ± 0.04
R43Q	6.8 ± 2.3	1.3 ± 0.3	15.0 ± 6.3	6.6 ± 0.09^c

Construct	P_Na+/P_K+	P_Na+/P_Li+	P_Cs+/P_Na+	pH₅₀
WT	8.0 ± 1.6	1.1 ± 0.2	29.9 ± 17.2	6.8 ± 0.03
F21A	1.0 ± 0.1^a	1.2 ± 0.2	0.9 ± 0.1^b	7.0 ± 0.04^a
F21L	1.0 ± 0.1^a	1.0 ± 0.1	1.4 ± 0.3^b	7.1 ± 0.05^c
F21W	4.1 ± 1.5^a	1.0 ± 0.3	10.4 ± 7.0^b	7.1 ± 0.08^a
A22G	1.1 ± 0.1^a	1.1 ± 0.1	1.1 ± 0.1^b	7.1 ± 0.02^a
A22L	1.0 ± 0.1^a	1.3 ± 0.3	0.9 ± 0.0^c	7.1 ± 0.01^a
S23A	9.0 ± 2.0	1.3 ± 0.2	40.4 ± 22.9	6.6 ± 0.01
S23E	8.3 ± 0.8	1.5 ± 0.3	46.2 ± 32.4	6.8 ± 0.01
S24A	9.8 ± 1.1	1.1 ± 0.1	44.2 ± 15.1	6.8 ± 0.1
S24E	10.7 ± 3.9^b	n.d.	40.3 ± 21.5	6.8 ± 0.02
S25A	5.6 ± 1.6^b	1.1 ± 0.2	16.6 ± 4.7	6.8 ± 0.07
S25E	1.5 ± 0.6^a	1.5 ± 0.5	2.0 ± 1.3^a	7.1 ± 0.1^a
T26C	0.9 ± 0.2^a	1.0 ± 0.3	1.0 ± 0.2^a	7.1 ± 0.03^a
T26E	1.1 ± 0.3^a	1.3 ± 0.5	1.1 ± 0.6^a	7.2 ± 0.05^a
T26V	0.9 ± 0.3^a	1.0 ± 0.5	1.1 ± 0.2^a	7.2 ± 0.04^a
T26S	5.7 ± 1.8^b	1.2 ± 0.4	9.7 ± 1.7^d	6.8 ± 0.04
H28A	0.8 ± 0.1^a	1.6 ± 0.3	1.1 ± 0.1^c	7.3 ± 0.06^a
G29A	1.1 ± 0.2^a	1.5 ± 0.6	1.6 ± 0.5 ^c	7.2 ± 0.04^a
I33A	1.0 ± 0.2^a	1.0 ± 0.4	1.1 ± 0.1 ^c	7.0 ± 0.03^a
K42Q	3.7 ± 0.3^a	0.7 ± 0.2	3.6 ± 1.7 ^c	6.8 ± 0.04
R43Q	6.8 ± 2.3	1.3 ± 0.3	15.0 ± 6.3	6.6 ± 0.09^c

Relative ion permeabilities and pH₅₀ values of WT mASIC1a and mutants containing amino acid substitutions in the pre-M1 region. Data are shown as mean ± SD with n ≥ 4, where n refers to the number of individual oocytes; n.d., not determined. Values for mutants were compared with WT using one-way ANOVA with Dunnett’s multiple comparisons test.

P < 0.0001.

P < 0.01.

P < 0.001.

P < 0.05.

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