| Construct . | Single-channel conductance (pS) . | n . |
|---|---|---|
| WT hENaC | 4.7 ± 1.0 | 6 |
| α(W0′F)βγ | 7.4 ± 3.1a | 5 |
| α(W0′F)β(W0′F)γ | 8.6 ± 1.0b | 6 |
| α(W4′A)βγ | 6.6 ± 1.0 | 4 |
| α(W4′A)β(T4′A)γ | 5.3 ± 2.2 | 7 |
| Construct . | Single-channel conductance (pS) . | n . |
|---|---|---|
| WT hENaC | 4.7 ± 1.0 | 6 |
| α(W0′F)βγ | 7.4 ± 3.1a | 5 |
| α(W0′F)β(W0′F)γ | 8.6 ± 1.0b | 6 |
| α(W4′A)βγ | 6.6 ± 1.0 | 4 |
| α(W4′A)β(T4′A)γ | 5.3 ± 2.2 | 7 |
WT and mutant hENaC RNA was injected into Xenopus oocytes, and single-channel conductances were determined in the cell-attached patch-clamp configuration with 140 mM extracellular Na+. Single-channel conductances are presented as mean ± SD and were compared with WT ENaC using one-way ANOVA with Dunnett’s multiple comparisons test.
P < 0.05.
P < 0.001.