| Construct . | IK/INa (−100 mV) . | ILi/INa (−100 mV) . | ICs/INa (−100 mV) . | n . |
|---|---|---|---|---|
| WT hENaC | −0.04 ± 0.04 | 1.2 ± 0.2 | −0.04 ± 0.04 | 7 |
| α(W-1′A)βγ | -0.02 ± 0.02 | 1.5 ± 0.4 | −0.04 ± 0.06 | 6 |
| α(W0′F)βγ | −0.02 ± 0.00 | 2.2 ± 0.5 | −0.03 ± 0.00 | 6 |
| α(W0′F)β(W0′F)γ | 0.2 ± 0.3a | 3.5 ± 1.3b | 0.03 ± 0.06 | 5 |
| α(W0′F)β(W0′F)γ(W0′F) | n.d. | n.d. | n.d. | |
| α(W4′A)βγ | 0.00 ± 0.02 | 1.7 ± 0.5 | 0.0 ± 0.1 | 6 |
| α(W4′A)β(T4′A)γ | 0.00 ± 0.06 | 2.0 ± 0.9 | −0.1 ± 0.1c | 3–4 |
| α(W4′A)β(T4′A)γ(T4′A) | 0.00 ± 0.02 | 1.5 ± 0.3 | −0.02 ± 0.01 | 4 |
| α(F8′A)βγ | −0.1 ± 0.1c | 0.9 ± 0.2 | −0.1 ± 0.1a | 5–6 |
| α(Y12′A)βγ | −0.03 ± 0.05 | 1.4 ± 0.5 | −0.04 ± 0.1 | 4–6 |
| α(W13′A)βγ | −0.00 ± 0.01 | 1.0 ± 0.5 | −0.01 ± 0.01 | 6 |
| α(W13′A)β(W13′A)γ | −0.00 ± 0.01 | 1.0 ± 0.7 | −0.00 ± 0.01 | 4–7 |
| α(W13′A)β(W13′A)γ(W13′A) | 0.02 ± 0.02 | 1.2 ± 0.2 | −0.01 ± 0.01 | 6–8 |
| α(F15′A)βγ | 0.01 ± 0.02 | 2.0 ± 0.3 | −0.01 ± 0.00 | 3 |
| α(F15′A)β(W15′A)γ | 0.01 ± 0.01 | 2.0 ± 0.5 | 0.01 ± 0.02 | 4–5 |
| α(F15′A)β(W15′A)γ(C13′A) | 0.00 ± 0.01 | 2.2 ± 0.8 | −0.03 ± 0.01 | 3 |
| α(F19′A)βγ | 0.04 ± 0.08a | 2.0 ± 1.0c | −0.01 ± 0.03 | 9–12 |
| α(Y22′A)βγ | 0.01 ± 0.01 | 2.6 ± 0.3d | 0.09 ± 0.1d | 3–5 |
| α(Y23′A)βγ | 0.05 ± 0.03c | 1.3 ± 0.2 | 0.03 ± 0.05 | 5 |
| αβ(F1′A)γ | 0.0 ± 0.0 | 1.3 ± 0.2 | −0.03 ± 0.04 | 3–4 |
| αβ(F7′A)γ | 0.0 ± 0.01 | 1.2 ± 0.4 | −0.00 ± 0.00 | 3–5 |
| αβ(F18′A)γ | 0.02 ± 0.01 | 1.4 ± 0.4 | 0.00 ± 0.02 | 3–5 |
| αβ(F22′A)γ | 0.01 ± 0.01 | 2.1 ± 0.2c | 0.01 ± 0.03 | 7–8 |
| αβγ(F3′A) | −0.02 ± 0.03 | 1.7 ± 0.3 | −0.00 ± 0.01 | 3–4 |
| αβγ(F20′A) | 0.01 ± 0.00 | 1.7 ± 0.7 | −0.00 ± 0.01 | 4–5 |
| αβγ(F24′A) | −0.0 ± 0.0 | 1.8 ± 0.47 | −0.01 ± 0.02 | 7–8 |
| αβγ(Y25′A) | 0.04 ± 0.05 | 1.4 ± 0.20 | −0.02 ± 0.01 | 3–5 |
| Construct . | IK/INa (−100 mV) . | ILi/INa (−100 mV) . | ICs/INa (−100 mV) . | n . |
|---|---|---|---|---|
| WT hENaC | −0.04 ± 0.04 | 1.2 ± 0.2 | −0.04 ± 0.04 | 7 |
| α(W-1′A)βγ | -0.02 ± 0.02 | 1.5 ± 0.4 | −0.04 ± 0.06 | 6 |
| α(W0′F)βγ | −0.02 ± 0.00 | 2.2 ± 0.5 | −0.03 ± 0.00 | 6 |
| α(W0′F)β(W0′F)γ | 0.2 ± 0.3a | 3.5 ± 1.3b | 0.03 ± 0.06 | 5 |
| α(W0′F)β(W0′F)γ(W0′F) | n.d. | n.d. | n.d. | |
| α(W4′A)βγ | 0.00 ± 0.02 | 1.7 ± 0.5 | 0.0 ± 0.1 | 6 |
| α(W4′A)β(T4′A)γ | 0.00 ± 0.06 | 2.0 ± 0.9 | −0.1 ± 0.1c | 3–4 |
| α(W4′A)β(T4′A)γ(T4′A) | 0.00 ± 0.02 | 1.5 ± 0.3 | −0.02 ± 0.01 | 4 |
| α(F8′A)βγ | −0.1 ± 0.1c | 0.9 ± 0.2 | −0.1 ± 0.1a | 5–6 |
| α(Y12′A)βγ | −0.03 ± 0.05 | 1.4 ± 0.5 | −0.04 ± 0.1 | 4–6 |
| α(W13′A)βγ | −0.00 ± 0.01 | 1.0 ± 0.5 | −0.01 ± 0.01 | 6 |
| α(W13′A)β(W13′A)γ | −0.00 ± 0.01 | 1.0 ± 0.7 | −0.00 ± 0.01 | 4–7 |
| α(W13′A)β(W13′A)γ(W13′A) | 0.02 ± 0.02 | 1.2 ± 0.2 | −0.01 ± 0.01 | 6–8 |
| α(F15′A)βγ | 0.01 ± 0.02 | 2.0 ± 0.3 | −0.01 ± 0.00 | 3 |
| α(F15′A)β(W15′A)γ | 0.01 ± 0.01 | 2.0 ± 0.5 | 0.01 ± 0.02 | 4–5 |
| α(F15′A)β(W15′A)γ(C13′A) | 0.00 ± 0.01 | 2.2 ± 0.8 | −0.03 ± 0.01 | 3 |
| α(F19′A)βγ | 0.04 ± 0.08a | 2.0 ± 1.0c | −0.01 ± 0.03 | 9–12 |
| α(Y22′A)βγ | 0.01 ± 0.01 | 2.6 ± 0.3d | 0.09 ± 0.1d | 3–5 |
| α(Y23′A)βγ | 0.05 ± 0.03c | 1.3 ± 0.2 | 0.03 ± 0.05 | 5 |
| αβ(F1′A)γ | 0.0 ± 0.0 | 1.3 ± 0.2 | −0.03 ± 0.04 | 3–4 |
| αβ(F7′A)γ | 0.0 ± 0.01 | 1.2 ± 0.4 | −0.00 ± 0.00 | 3–5 |
| αβ(F18′A)γ | 0.02 ± 0.01 | 1.4 ± 0.4 | 0.00 ± 0.02 | 3–5 |
| αβ(F22′A)γ | 0.01 ± 0.01 | 2.1 ± 0.2c | 0.01 ± 0.03 | 7–8 |
| αβγ(F3′A) | −0.02 ± 0.03 | 1.7 ± 0.3 | −0.00 ± 0.01 | 3–4 |
| αβγ(F20′A) | 0.01 ± 0.00 | 1.7 ± 0.7 | −0.00 ± 0.01 | 4–5 |
| αβγ(F24′A) | −0.0 ± 0.0 | 1.8 ± 0.47 | −0.01 ± 0.02 | 7–8 |
| αβγ(Y25′A) | 0.04 ± 0.05 | 1.4 ± 0.20 | −0.02 ± 0.01 | 3–5 |
Relative amiloride-sensitive currents determined for WT ENaC and hENaC mutants containing the designated amino acid substitutions at positions with aromatic side chain. Data are shown as mean ± SD; n.d., not determined. n refers to the number of individual oocytes. Values for mutants were compared with WT hENaC using one-way ANOVA with Dunnett’s multiple comparisons test.
P < 0.01.
P < 0.0001.
P < 0.05.
P < 0.001.