| Construct . | INa+ (μA) . |
|---|---|
| hENaC WT | −12.3 ± 4.4 |
| α(W0′F)βγ | −2.1 ± 0.4a |
| α(W0′F)β(W0′F)γ | −0.7 ± 0.4a |
| α(W0′F)β(W0′F)γ(W0′F) | n.d. |
| α(W4′A)βγ | −5.7 ± 2.6b |
| α(W4′A)β(T4′A)γ | −3.4 ± 1.7b |
| α(W4′A)β(T4′A)γ(T4′A) | −14.0 ± 5.3 |
| Construct . | INa+ (μA) . |
|---|---|
| hENaC WT | −12.3 ± 4.4 |
| α(W0′F)βγ | −2.1 ± 0.4a |
| α(W0′F)β(W0′F)γ | −0.7 ± 0.4a |
| α(W0′F)β(W0′F)γ(W0′F) | n.d. |
| α(W4′A)βγ | −5.7 ± 2.6b |
| α(W4′A)β(T4′A)γ | −3.4 ± 1.7b |
| α(W4′A)β(T4′A)γ(T4′A) | −14.0 ± 5.3 |
Xenopus oocytes were injected with 5 ng RNA (hENaC WT, α(W4′A)βγ and α(W4′A)β(T4′A)γ) or 50 ng RNA (α(W0′F)βγ, α(W0′F)β(W0′F)γ, and α(W0′F)β(W0′F)γ(W0′F)) and incubated for 1–3 d. Values are presented as mean ± SD. n.d., not determined. Values for the mutants were compared with WT and analyzed using one-way ANOVA with Dunnett’s multiple comparisons test.
P < 0.0001.
P < 0.01.