Table I.

Initial conditions

Species Amount Rationale 
R 500 µm−2 From fluorescencea 
G 40 µm−2 To fit concentration–response curve of currenta 
PLC 10 µm−2 To fit concentration–response curve of currenta 
PI 140,000 µm−2b Xu et al. (2003); Suh et al. (2004)  
PIP 4,000 µm−2 Suh et al. (2004); ratio to PIP2 as in Willars et al. (1998); Winks et al. (2005)  
PIP2 5,000 µm−2 To have 50% PH probes at the membrane, as in Horowitz et al. (2005), similar to Xu et al. (2003)  
PH domainsc (membrane) 3,000 µm−2 From fluorescencea, similar to Xu et al. (2003)  
PH domainsc (cytosol) 3 µM 1 µM free, 2 µM PH_IP3; see Fig. S3, similar to Xu et al. (2003)  
KCNQ2/3 channels 4 µm−2 From whole cell current, open probability, and single-channel conductance; consistent with Zaika et al. (2008)  
IP3 0.16 µM Fink et al. (1999); Xu et al. (2003); Winks et al. (2005)  
Species Amount Rationale 
R 500 µm−2 From fluorescencea 
G 40 µm−2 To fit concentration–response curve of currenta 
PLC 10 µm−2 To fit concentration–response curve of currenta 
PI 140,000 µm−2b Xu et al. (2003); Suh et al. (2004)  
PIP 4,000 µm−2 Suh et al. (2004); ratio to PIP2 as in Willars et al. (1998); Winks et al. (2005)  
PIP2 5,000 µm−2 To have 50% PH probes at the membrane, as in Horowitz et al. (2005), similar to Xu et al. (2003)  
PH domainsc (membrane) 3,000 µm−2 From fluorescencea, similar to Xu et al. (2003)  
PH domainsc (cytosol) 3 µM 1 µM free, 2 µM PH_IP3; see Fig. S3, similar to Xu et al. (2003)  
KCNQ2/3 channels 4 µm−2 From whole cell current, open probability, and single-channel conductance; consistent with Zaika et al. (2008)  
IP3 0.16 µM Fink et al. (1999); Xu et al. (2003); Winks et al. (2005)  
a

See Falkenburger et al. (2010).

b

Amount of PI is clamped at 140,000 µm−2.

c

Not present in all experiments.

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