Parameters for block and allosterically coupled changes in activation used in simulations
| Complete state-independent block | |||
| Scheme 2a | |||
| Block parameters (rates in s−1) | Allosterically altered parameters | ||
| fc(0) | 0–5,000 (z = 0.05 e) | ||
| bc(0) | 350 (z = −0.05 e) | ||
| fo(0) | 0–5,000 (z = 0.05 e) | ||
| bo(0) | 350 (z = −0.05 e) | ||
| Scheme 2b | Closed channel block is voltage independent. To maintain microscopic reversibility, voltage dependence of CB–OB transitions is allosterically influenced. | ||
| fc(0) | 0–5,000 (z = 0.0 e) | z (δ(0)) | 0.262 e |
| bc(0) | 350 (z = −0.0 e) | z (γ(0)) | −0.138 e |
| fo(0) | 0–5,000 (z = 0.05 e) | ||
| bo(0) | 350 (z = −0.05 e) | ||
| Scheme 3a | Block dependent on movement of at least one voltage sensor | ||
| fc(0) | 0–5,000 (z = 0.05 e) | ||
| bc(0) | 350 (z = −0.05 e) | ||
| fo(0) | 0–5,000 (z = 0.05 e) | ||
| bo(0) | 350 (z = −0.05 e) | ||
| Scheme 3b | Block dependent on movement of at least one voltage sensor, but closed-channel block is voltage independent. To maintain microscopic reversibility, voltage dependence of CB–OB transitions is allosterically influenced. | ||
| fc(0) | 0–5,000 (z = 0.0 e) | z (δ(0))o | 0.262 e |
| bc(0) | 350 (z = −0.0 e) | z (γ(0))o | −0.138 e |
| fo(0) | 0–5,000 (z = 0.05 e) | ||
| bo(0) | 350 (z = −0.05 e) | ||
| Scheme 1a | Open-channel block | ||
| fon(0) | 0–5,000 (z = 0.05 e) | ||
| bo(0) | 350 (z = −0.05 e) | ||
| Complete state-independent block | |||
| Scheme 2a | |||
| Block parameters (rates in s−1) | Allosterically altered parameters | ||
| fc(0) | 0–5,000 (z = 0.05 e) | ||
| bc(0) | 350 (z = −0.05 e) | ||
| fo(0) | 0–5,000 (z = 0.05 e) | ||
| bo(0) | 350 (z = −0.05 e) | ||
| Scheme 2b | Closed channel block is voltage independent. To maintain microscopic reversibility, voltage dependence of CB–OB transitions is allosterically influenced. | ||
| fc(0) | 0–5,000 (z = 0.0 e) | z (δ(0)) | 0.262 e |
| bc(0) | 350 (z = −0.0 e) | z (γ(0)) | −0.138 e |
| fo(0) | 0–5,000 (z = 0.05 e) | ||
| bo(0) | 350 (z = −0.05 e) | ||
| Scheme 3a | Block dependent on movement of at least one voltage sensor | ||
| fc(0) | 0–5,000 (z = 0.05 e) | ||
| bc(0) | 350 (z = −0.05 e) | ||
| fo(0) | 0–5,000 (z = 0.05 e) | ||
| bo(0) | 350 (z = −0.05 e) | ||
| Scheme 3b | Block dependent on movement of at least one voltage sensor, but closed-channel block is voltage independent. To maintain microscopic reversibility, voltage dependence of CB–OB transitions is allosterically influenced. | ||
| fc(0) | 0–5,000 (z = 0.0 e) | z (δ(0))o | 0.262 e |
| bc(0) | 350 (z = −0.0 e) | z (γ(0))o | −0.138 e |
| fo(0) | 0–5,000 (z = 0.05 e) | ||
| bo(0) | 350 (z = −0.05 e) | ||
| Scheme 1a | Open-channel block | ||
| fon(0) | 0–5,000 (z = 0.05 e) | ||
| bo(0) | 350 (z = −0.05 e) | ||