Table I. Sequence alignment a and... | Rockefeller University Press

Table I.

Sequence alignment^a and effects of MTSET on channels with engineered cysteines^b

Channel	Segment		No.	1	11	21
KcsA	M1	o	23	ALHWRAAGAA	TVLLVIVLLA	GSYLAVLAER
K_v1.2	S5	o	322	KASMRELGLL	IFFLFIGVIL	FSSAVYFAEA
Ca_v2.1	IS5	1o	220	MKAMIPLLQI	GLLLFFAILI	FAIIGLEFYM
	IIS5	2o	608	LNSMKSIISL	LFLLFLFIVV	FALLGMQLFG
	IIIS5	3o	1380	VNSLKNVFNI	LIVYMLFMFI	FAVVAVQLFK
	IVS5	4o	1695	VQSFKALPYV	CLLIAMLFFI	YAIIGMQVFG
Pore-facing position				* *	* *
				1	11	21	31
KcsA	M2	i	86	LWGRLVAVVV	MVAGITSFGL	VTAALATWFV	GREQ
K_v1.2	S6	i	385	IGGKIVGSLC	AIAGVLTIAL	PVPVIVSNFN	YFYH
Ca_v2.1	IS6	1i	336	TWNWLYFIPL	IIIGSFFMLN	LVLGVLSGEF	AKER
	IIS6	2i	690	MVFSIYFIVL	TLFGNYTLLN	VFLAIAVDNL	ANAQ
	IIIS6	3i	1485	MEMSIFYVVY	FVVFPFFFVN	IFVALIIITF	QEQG
	IVS6	4i	1785	EFAYFYFVSF	IFLCSFLMLN	LFVAVIMDNF	EYLT
Na_v1.4	IVS6	4i	1565	SIGICFFCSY	IIISFLIVVN	MYIAIILENF	NVAT
Pore-facing position					* * **
Cytoplasm-facing position						* *	*

Channel	Segment		No.	1	11	21
KcsA	M1	o	23	ALHWRAAGAA	TVLLVIVLLA	GSYLAVLAER
K_v1.2	S5	o	322	KASMRELGLL	IFFLFIGVIL	FSSAVYFAEA
Ca_v2.1	IS5	1o	220	MKAMIPLLQI	GLLLFFAILI	FAIIGLEFYM
	IIS5	2o	608	LNSMKSIISL	LFLLFLFIVV	FALLGMQLFG
	IIIS5	3o	1380	VNSLKNVFNI	LIVYMLFMFI	FAVVAVQLFK
	IVS5	4o	1695	VQSFKALPYV	CLLIAMLFFI	YAIIGMQVFG
Pore-facing position				* *	* *
				1	11	21	31
KcsA	M2	i	86	LWGRLVAVVV	MVAGITSFGL	VTAALATWFV	GREQ
K_v1.2	S6	i	385	IGGKIVGSLC	AIAGVLTIAL	PVPVIVSNFN	YFYH
Ca_v2.1	IS6	1i	336	TWNWLYFIPL	IIIGSFFMLN	LVLGVLSGEF	AKER
	IIS6	2i	690	MVFSIYFIVL	TLFGNYTLLN	VFLAIAVDNL	ANAQ
	IIIS6	3i	1485	MEMSIFYVVY	FVVFPFFFVN	IFVALIIITF	QEQG
	IVS6	4i	1785	EFAYFYFVSF	IFLCSFLMLN	LFVAVIMDNF	EYLT
Na_v1.4	IVS6	4i	1565	SIGICFFCSY	IIISFLIVVN	MYIAIILENF	NVAT
Pore-facing position					* * **
Cytoplasm-facing position						* *	*

Data for Na_v1.4 are from (Sunami et al., 2004).

Shown alignments of S5 (Huber et al., 2000) and S6 (Zhorov et al., 2001) segments were used to build the Ca_v2.1 models.

Bold-oblique and underlined characters indicate positions where MTSET inhibits channels with engineered cysteines ≥30 and <30%, respectively (Zhen et al., 2005).

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