Rate constants
| Parameter | Value | Units | Comment |
| α | 100 | Allosteric constant (cooperativity factor); Cortés and Palacios (1986); Vannucchi and Goldman-Rakic (1991) | |
| KL1 | 2 | µM | KL2 * α, similar to EC50 for M1R-Y-C FRET |
| KL2 | 0.02 | µM | From EC50 for Oxo-M on M1R/Gβ FRET |
| KG1 | 25,500 | µm−2 | α * KG2 |
| KG2 | 255 | µm−2 | kr_G2/kf_G2 |
| kf_L1 | 2.8 | µM−1 s−1 | kr_L1/KL1 |
| kr_L1 | 5.5 | s−1 | From M1R-Y-C FRET recovery |
| kf_L2 | 2.8 | µM−1 s−1 | As kf_L1 |
| kr_L2 | 5.5 × 10−2 | s−1 | kf_L1 * KL2 |
| kf_G1 | 2.7 × 10−4 | µm2 s−1 | Not well-constrained |
| kr_G1 | 6.8 | s−1 | kf_G1 * KG1 |
| kf_G2 | 2.7 × 10−3 | µm2 s−1 | From M1R/Gβ FRET onset |
| kr_G2 | 0.68 | s−1 | From M1R/Gβ FRET recovery |
| k_NX_RLG | 0.65 | s−1 | From Gα/Gβ FRET onset, fits Gα/PLC FRET onset |
| k_NX_G | 1.5 × 10−5 | s−1 | From Turcotte model, d1a |
| k_NX_P | 4.7 | s−1 | From Turcotte model, d4b |
| k_GTPase1 | 0.026 | s−1 | From Gα/Gβ FRET recovery |
| k_GTPase2 | 15.0 | s−1 | From Turcotte model, p4c |
| k_PLCassoc | 1 | µm2 s−1 | Not rate-limiting |
| k_PLCdiss | 0.71 | s−1 | From Gα-PLC FRET recovery |
| k_reconst | 1 | µm2 s−1 | Reconstitution of Gαβγ trimers, affects timing if <1.0 |
| Parameter | Value | Units | Comment |
| α | 100 | Allosteric constant (cooperativity factor); Cortés and Palacios (1986); Vannucchi and Goldman-Rakic (1991) | |
| KL1 | 2 | µM | KL2 * α, similar to EC50 for M1R-Y-C FRET |
| KL2 | 0.02 | µM | From EC50 for Oxo-M on M1R/Gβ FRET |
| KG1 | 25,500 | µm−2 | α * KG2 |
| KG2 | 255 | µm−2 | kr_G2/kf_G2 |
| kf_L1 | 2.8 | µM−1 s−1 | kr_L1/KL1 |
| kr_L1 | 5.5 | s−1 | From M1R-Y-C FRET recovery |
| kf_L2 | 2.8 | µM−1 s−1 | As kf_L1 |
| kr_L2 | 5.5 × 10−2 | s−1 | kf_L1 * KL2 |
| kf_G1 | 2.7 × 10−4 | µm2 s−1 | Not well-constrained |
| kr_G1 | 6.8 | s−1 | kf_G1 * KG1 |
| kf_G2 | 2.7 × 10−3 | µm2 s−1 | From M1R/Gβ FRET onset |
| kr_G2 | 0.68 | s−1 | From M1R/Gβ FRET recovery |
| k_NX_RLG | 0.65 | s−1 | From Gα/Gβ FRET onset, fits Gα/PLC FRET onset |
| k_NX_G | 1.5 × 10−5 | s−1 | From Turcotte model, d1a |
| k_NX_P | 4.7 | s−1 | From Turcotte model, d4b |
| k_GTPase1 | 0.026 | s−1 | From Gα/Gβ FRET recovery |
| k_GTPase2 | 15.0 | s−1 | From Turcotte model, p4c |
| k_PLCassoc | 1 | µm2 s−1 | Not rate-limiting |
| k_PLCdiss | 0.71 | s−1 | From Gα-PLC FRET recovery |
| k_reconst | 1 | µm2 s−1 | Reconstitution of Gαβγ trimers, affects timing if <1.0 |