Initial conditions
| Species | Density | Source |
| µm−2 | ||
| R (endogenous) | 1 | From ratio of endogenous R to G |
| R (M1R-YFP) | 3,000 | From fluorescence |
| R (M1R, selected for FRET) | 3,000 | To reproduce Gα/Gβ FRET onset |
| R (M1R, current measurements) | 500 | Arithmetic mean of all cells (flow cytometry) |
| G protein (endogenous) | 40 | To fit concentration-response curve of current |
| G protein (overexpressed) | 3,000 | From fluorescence |
| PLCβ1 (endogenous) | 3 | From western blot |
| PLCβ1 (overexpressed) | 3,000 | From fluorescence |
| PLC (total endogenous) | 10 | To fit concentration-response curve of current |
| PH domains (membrane) | 3,000 | From fluorescence, consistent with FRETr |
| Species | Density | Source |
| µm−2 | ||
| R (endogenous) | 1 | From ratio of endogenous R to G |
| R (M1R-YFP) | 3,000 | From fluorescence |
| R (M1R, selected for FRET) | 3,000 | To reproduce Gα/Gβ FRET onset |
| R (M1R, current measurements) | 500 | Arithmetic mean of all cells (flow cytometry) |
| G protein (endogenous) | 40 | To fit concentration-response curve of current |
| G protein (overexpressed) | 3,000 | From fluorescence |
| PLCβ1 (endogenous) | 3 | From western blot |
| PLCβ1 (overexpressed) | 3,000 | From fluorescence |
| PLC (total endogenous) | 10 | To fit concentration-response curve of current |
| PH domains (membrane) | 3,000 | From fluorescence, consistent with FRETr |