Table I. Absorbance of CFP and YFP... | Rockefeller University Press

Table I.

Absorbance of CFP and YFP solutions

	ε	A	C
	mM⁻¹		µM
CFP, 280 nm	25.9^a	16.7 ± 0.7	646 ± 27
CFP, 440 nm	26^b	16.0 ± 0.9	617 ± 33
YFP, 280 nm	23.4^a	10.8 ± 0.3	460 ± 11
YFP, 515 nm	84^b	30.5 ± 0.4	363 ± 5
YFP, denatured	44^c	13.8 ± 1.3	313 ± 31

	ε	A	C
	mM⁻¹		µM
CFP, 280 nm	25.9^a	16.7 ± 0.7	646 ± 27
CFP, 440 nm	26^b	16.0 ± 0.9	617 ± 33
YFP, 280 nm	23.4^a	10.8 ± 0.3	460 ± 11
YFP, 515 nm	84^b	30.5 ± 0.4	363 ± 5
YFP, denatured	44^c	13.8 ± 1.3	313 ± 31

Measurements on three different days, mean ± SEM. ε, published extinction coefficients; A, measured absorbance of the stock solution; C, protein concentration of the stock solution estimated as A/ε.

Calculated from molar extinction coefficients for tyrosine (1.49 mM⁻¹) and tryptophan (5.5 mM⁻¹; from Oregon Medical Laser Center).

From Patterson et al. (2001).

From Ward (2006).

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