Table I.

Comparison of unitary current amplitudes for wild-type and mutant channels at [K+] = 1 M (current represented in pA as means ± SEM with n = 3–9)

 −60 mV +60 mV 
 pH 5.5 pH 7.4 pH 8.5 pH 5.5 pH 7.4 pH 8.5 
Wild-type 5.3 ± 0.2 9.3 ± 0.4 11 ± 0.3 13 ± 0.4 16 ± 0.4 17 ± 0.5 
E636Q 6 ± 1 8 ± 0.6 8.7 ± 0.6 12 ± 0.5 13 ± 0.7 14 ± 0.9 
D646N 5.5 ± 0.2 7 ± 0.2 7.8 ± 0.1 11 ± 0.5 12 ± 0.3 13 ± 0.5 
E636Q/D646N 5 ± 0.1 5.4 ± 0.2 5.7 ± 0.2 8.2 ± 0.2 8.9 ± 0.2 9.3 ± 0.4 
 −60 mV +60 mV 
 pH 5.5 pH 7.4 pH 8.5 pH 5.5 pH 7.4 pH 8.5 
Wild-type 5.3 ± 0.2 9.3 ± 0.4 11 ± 0.3 13 ± 0.4 16 ± 0.4 17 ± 0.5 
E636Q 6 ± 1 8 ± 0.6 8.7 ± 0.6 12 ± 0.5 13 ± 0.7 14 ± 0.9 
D646N 5.5 ± 0.2 7 ± 0.2 7.8 ± 0.1 11 ± 0.5 12 ± 0.3 13 ± 0.5 
E636Q/D646N 5 ± 0.1 5.4 ± 0.2 5.7 ± 0.2 8.2 ± 0.2 8.9 ± 0.2 9.3 ± 0.4 

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