Residue 18 coupling potential analysis
| Mutant | Tau on, msec | Tau recovery, msec | ΔG(τon), kT | ΔG(τrecov), kT | On slope, ΔkT/(+) | Recov slope, ΔkT/(+) |
| V135K/R18 | 22.8 ± 1.3 (n = 12)a | 134.8 ± 4.4 (n = 12) | 3.13 ± 0.06 | 4.90 ± 0.03 | ||
| V135K/R18A | 61.8 ± 0.89 (n = 4)a | 150.8 ± 2.8 (n = 4)a | 4.12 ± 0.01 | 5.02 ± 0.02 | −0.96 | −0.22 |
| V135K/R18E | 117.6 ± 8.6 (n = 3)b | 168 ± 21 (n = 3) | 4.80 ± 0.07 | 5.12 ± 0.13 | ||
| V135/R18 | 29.5 ± 0.16 (n = 17) | 138.8 ± 4.9 (n = 27) | 3.38 ± 0.01 | 4.93 ± 0.04 | ||
| V135/R18A | 98 ± 3.8 (n = 12)a | 123 ± 5.6 (n = 5)b | 4.58 ± 0.04 | 4.81 ± 0.05 | −1.28 | −0.05 |
| V135/R18E | 401.6 ± 15.7 (n = 16)a | 127.4 ± 5.6 (n = 15)a | 6.00 ± 0.04 | 4.85 ± 0.04 | ||
| V135D/R18 | 39.2 ± 1.5 (n = 3)a | 166.4 ± 6.5 (n = 3)a | 3.67 ± 0.04 | 5.11 ± 0.04 | ||
| V135D/R18A | 258 ± 15.2 (n = 10)a | 133.3 ± 7 (n = 9)b | 5.55 ± 0.06 | 4.89 ± 0.05 | −1.63 | −0.20 |
| V135D/R18E | 1006 ± 75 (n = 10)a | 116.8 ± 2.7 (n = 10)b | 6.91 ± 0.07 | 4.76 ± 0.02 | ||
| KKK/R18 | 321 ± 9.5 (n = 16)a | 286.5 ± 12.4 (n = 13)a | 5.77 ± 0.03 | 5.66 ± 0.04 | ||
| KKK/R18A | 465.7 ± 15.4 (n = 9)a | 328.2 ± 14.2 (n = 4) | 6.14 ± 0.03 | 5.79 ± 0.04 | −0.14 | 0.00 |
| KKK/R18E | 428 ± 16.7 (n = 6)a | 289 ± 13 (n = 6) | 6.06 ± 0.04 | 5.67 ± 0.04 | ||
| AAA/R18 | 44.5 ± 0.8 (n = 3)a | 152.5 ± 7.4 (n = 3)a | 3.80 ± 0.02 | 5.03 ± 0.05 | ||
| AAA/R18A | 101.8 ± 4 (n = 6)a | 208.1 ± 9.9 (n = 6)a | 4.62 ± 0.04 | 5.33 ± 0.05 | −0.53 | −0.13 |
| AAA/R18E | 113.9 ± 4 (n = 6)a | 203.4 ± 4.5 (n = 6)b | 4.74 ± 0.04 | 5.32 ± 0.02 | ||
| EDE/R18 | 29.5 ± 0.16 (n = 17) | 138.8 ± 4.9 (n = 27) | 3.38 ± 0.01 | 4.93 ± 0.04 | ||
| EDE/R18A | 98 ± 3.8 (n = 12)a | 123 ± 5.6 (n = 5)b | 4.58 ± 0.04 | 4.81 ± 0.05 | −1.28 | −0.05 |
| EDE/R18E | 401.6 ± 15.7 (n = 16)a | 127.4 ± 5.6 (n = 15)a | 6.00 ± 0.04 | 4.85 ± 0.04 |
| Mutant | Tau on, msec | Tau recovery, msec | ΔG(τon), kT | ΔG(τrecov), kT | On slope, ΔkT/(+) | Recov slope, ΔkT/(+) |
| V135K/R18 | 22.8 ± 1.3 (n = 12)a | 134.8 ± 4.4 (n = 12) | 3.13 ± 0.06 | 4.90 ± 0.03 | ||
| V135K/R18A | 61.8 ± 0.89 (n = 4)a | 150.8 ± 2.8 (n = 4)a | 4.12 ± 0.01 | 5.02 ± 0.02 | −0.96 | −0.22 |
| V135K/R18E | 117.6 ± 8.6 (n = 3)b | 168 ± 21 (n = 3) | 4.80 ± 0.07 | 5.12 ± 0.13 | ||
| V135/R18 | 29.5 ± 0.16 (n = 17) | 138.8 ± 4.9 (n = 27) | 3.38 ± 0.01 | 4.93 ± 0.04 | ||
| V135/R18A | 98 ± 3.8 (n = 12)a | 123 ± 5.6 (n = 5)b | 4.58 ± 0.04 | 4.81 ± 0.05 | −1.28 | −0.05 |
| V135/R18E | 401.6 ± 15.7 (n = 16)a | 127.4 ± 5.6 (n = 15)a | 6.00 ± 0.04 | 4.85 ± 0.04 | ||
| V135D/R18 | 39.2 ± 1.5 (n = 3)a | 166.4 ± 6.5 (n = 3)a | 3.67 ± 0.04 | 5.11 ± 0.04 | ||
| V135D/R18A | 258 ± 15.2 (n = 10)a | 133.3 ± 7 (n = 9)b | 5.55 ± 0.06 | 4.89 ± 0.05 | −1.63 | −0.20 |
| V135D/R18E | 1006 ± 75 (n = 10)a | 116.8 ± 2.7 (n = 10)b | 6.91 ± 0.07 | 4.76 ± 0.02 | ||
| KKK/R18 | 321 ± 9.5 (n = 16)a | 286.5 ± 12.4 (n = 13)a | 5.77 ± 0.03 | 5.66 ± 0.04 | ||
| KKK/R18A | 465.7 ± 15.4 (n = 9)a | 328.2 ± 14.2 (n = 4) | 6.14 ± 0.03 | 5.79 ± 0.04 | −0.14 | 0.00 |
| KKK/R18E | 428 ± 16.7 (n = 6)a | 289 ± 13 (n = 6) | 6.06 ± 0.04 | 5.67 ± 0.04 | ||
| AAA/R18 | 44.5 ± 0.8 (n = 3)a | 152.5 ± 7.4 (n = 3)a | 3.80 ± 0.02 | 5.03 ± 0.05 | ||
| AAA/R18A | 101.8 ± 4 (n = 6)a | 208.1 ± 9.9 (n = 6)a | 4.62 ± 0.04 | 5.33 ± 0.05 | −0.53 | −0.13 |
| AAA/R18E | 113.9 ± 4 (n = 6)a | 203.4 ± 4.5 (n = 6)b | 4.74 ± 0.04 | 5.32 ± 0.02 | ||
| EDE/R18 | 29.5 ± 0.16 (n = 17) | 138.8 ± 4.9 (n = 27) | 3.38 ± 0.01 | 4.93 ± 0.04 | ||
| EDE/R18A | 98 ± 3.8 (n = 12)a | 123 ± 5.6 (n = 5)b | 4.58 ± 0.04 | 4.81 ± 0.05 | −1.28 | −0.05 |
| EDE/R18E | 401.6 ± 15.7 (n = 16)a | 127.4 ± 5.6 (n = 15)a | 6.00 ± 0.04 | 4.85 ± 0.04 |
aP < 0.01 and bP < 0.05. R18 mutants compared to V135K or EDE(161-3)KKK parent constructs, respectively; R18 plus EDE(161-3)KKK or V135K constructs compared to wild type.