Mutant cycle analysis
| Mutant | Tau on, msec | Tau recovery, msec | Ω (On) | Ω (Recov) |
| V135K | 22.8 ± 1.3 (n = 12)a | 134.8 ± 4.4 (n = 12) | ||
| V135K/R18A | 61.8 ± 0.89 (n = 4)a | 150.8 ± 2.8 (n = 4)a | 1.6 ± 0.1 | 1.2 ± 0.1 |
| V135K/D19A | 34.3 ± 0.97 (n = 4)a | 177.2 ± 4.8 (n = 4)a | 1.5 ± 0.1 | 1.3 ± 0.1 |
| V135K/R26A | 27.3 ± 0.8 (n = 7)a | 94.4 ± 2.9 (n = 7)a | 1.2 ± 0.1 | 1.0 ± 0.1 |
| V135K/R30A | 33.2 ± 0.8 (n = 6)a | 92.5 ± 2.1 (n = 5)a | 1.1 ± 0.1 | 1.1 ± 0.1 |
| V135K/R38A | 33.4 ± 1.4 (n = 5)a | 110.4 ± 4.4 (n = 5)a | 1.0 ± 0.1 | 1.0 ± 0.1 |
| V135K/K42A | 33.3 ± 2.2 (n = 5)a | 105 ± 10 (n = 3) | 1.0 ± 0.1 | 1.0 ± 0.1 |
| EDE/KKK | 321 ± 9.5 (n = 16)a | 286.5 ± 12.4 (n = 13)a | ||
| KKK/R18A | 465.7 ± 15.4 (n = 9)a | 328.2 ± 14.2 (n = 4) | 2.3 ± 0.2b | 1.2 ± 0.1 |
| KKK/D19A | 367.9 ± 15 (n = 7)a | 318 ± 13.7 (n = 8)b | 1.1 ± 0.1 | 1.5 ± 0.1 |
| KKK/R26A | 345 ± 7.6 (n = 3) | 286.3 ± 13.7 (n = 3) | 1.5 ± 0.1 | 1.3 ± 0.1 |
| KKK/R30A | 402.4 ± 14 (n = 6)a | 264.25 ± 13 (n = 6) | 1.3 ± 0.1 | 1.3 ± 0.1 |
| KKK/R38A | 353 ± 9 (n = 8)a | 277.4 ± 12.7 (n = 8) | 1.4 ± 0.1 | 1.2 ± 0.1 |
| KKK/K42A | 349.7 ± 15.5 (n = 5) | 309.7 ± 20 (n = 5) | 1.3 ± 0.1 | 1.4 ± 0.1 |
| Mutant | Tau on, msec | Tau recovery, msec | Ω (On) | Ω (Recov) |
| V135K | 22.8 ± 1.3 (n = 12)a | 134.8 ± 4.4 (n = 12) | ||
| V135K/R18A | 61.8 ± 0.89 (n = 4)a | 150.8 ± 2.8 (n = 4)a | 1.6 ± 0.1 | 1.2 ± 0.1 |
| V135K/D19A | 34.3 ± 0.97 (n = 4)a | 177.2 ± 4.8 (n = 4)a | 1.5 ± 0.1 | 1.3 ± 0.1 |
| V135K/R26A | 27.3 ± 0.8 (n = 7)a | 94.4 ± 2.9 (n = 7)a | 1.2 ± 0.1 | 1.0 ± 0.1 |
| V135K/R30A | 33.2 ± 0.8 (n = 6)a | 92.5 ± 2.1 (n = 5)a | 1.1 ± 0.1 | 1.1 ± 0.1 |
| V135K/R38A | 33.4 ± 1.4 (n = 5)a | 110.4 ± 4.4 (n = 5)a | 1.0 ± 0.1 | 1.0 ± 0.1 |
| V135K/K42A | 33.3 ± 2.2 (n = 5)a | 105 ± 10 (n = 3) | 1.0 ± 0.1 | 1.0 ± 0.1 |
| EDE/KKK | 321 ± 9.5 (n = 16)a | 286.5 ± 12.4 (n = 13)a | ||
| KKK/R18A | 465.7 ± 15.4 (n = 9)a | 328.2 ± 14.2 (n = 4) | 2.3 ± 0.2b | 1.2 ± 0.1 |
| KKK/D19A | 367.9 ± 15 (n = 7)a | 318 ± 13.7 (n = 8)b | 1.1 ± 0.1 | 1.5 ± 0.1 |
| KKK/R26A | 345 ± 7.6 (n = 3) | 286.3 ± 13.7 (n = 3) | 1.5 ± 0.1 | 1.3 ± 0.1 |
| KKK/R30A | 402.4 ± 14 (n = 6)a | 264.25 ± 13 (n = 6) | 1.3 ± 0.1 | 1.3 ± 0.1 |
| KKK/R38A | 353 ± 9 (n = 8)a | 277.4 ± 12.7 (n = 8) | 1.4 ± 0.1 | 1.2 ± 0.1 |
| KKK/K42A | 349.7 ± 15.5 (n = 5) | 309.7 ± 20 (n = 5) | 1.3 ± 0.1 | 1.4 ± 0.1 |
aP < 0.01 and bP < 0.05. Mutants compared to V135K or EDE(161-3)KKK parent constructs, respectively; EDE(161-3)KKK or V135K constructs compared to wild type.