TABLE II. Polar region charge-changing... | Rockefeller University Press

TABLE II.

Polar region charge-changing point mutations

				Linear energy analysis
Mutant	Tau on, msec	Tau recovery, msec	K_eq	ΔΔG(τ_on), kT	ΔΔG(K_eq), kT
WT	29.5 ± 0.16 (n = 17)	138.8 ± 4.9 (n = 27)	0.21 ± 0.01
R18A	98 ± 3.8 (n = 12)^a	123 ± 5.6 (n = 5)^b	0.80 ± 0.05^a	1.20 ± 0.04	1.32 ± 0.07
R18E	401.6 ± 15.7 (n = 16)^a	127.4 ± 5.6 (n = 15)^a	3.15 ± 0.18^a	2.61 ± 0.04	2.70 ± 0.07
D19A	29.6 ± 0.9 (n = 7)	232.4 ± 12 (n = 8)^a	0.13 ± 0.01^a	0.00 ± 0.03	−0.51 ± 0.07
D19K	22.5 ± 0.8 (n = 7)^a	355.5 ± 6.2 (n = 5)^a	0.06 ± 0.003^a	−0.27 ± 0.04	−1.21 ± 0.05
R26A	47.6 ± 3.3 (n = 9)^a	106 ± 8 (n = 9)^a	0.45 ± 0.05^a	0.48 ± 0.07	0.75 ± 0.11
R30A	47.6 ± 2.3 (n = 9)^a	100.7 ± 4.2 (n = 9)^a	0.47 ± 0.03^a	0.48 ± 0.05	0.80 ± 0.07
R38A	42.3 ± 1.3 (n = 11)^a	116 ± 6.5 (n = 8)^a	0.36 ± 0.02^a	0.36 ± 0.03	0.54 ± 0.07
K42A	41.6 ± 2.4 (n = 10)^a	103.9 ± 5.1 (n = 10)^a	0.40 ± 0.03^a	0.34 ± 0.06	0.63 ± 0.08
E45A	26.8 ± 2.6 (n = 4)	106.4 ± 12 (n = 4)	0.25 ± 0.04	−0.10 ± 0.10	0.17 ± 0.15
E46A	21.2 ± 0.6 (n = 8)^a	152.5 ± 5.9 (n = 8)^b	0.14 ± 0.01^a	−0.33 ± 0.03	−0.42 ± 0.06
E46K	31.3 ± 1.3 (n = 7)	175.5 ± 0.8 (n = 7)^a	0.18 ± 0.01^a	0.06 ± 0.04	−0.17 ± 0.05
D47A	40 ± 1.5 (n = 6)^a	117 ± 2.5 (n = 5)^a	0.34 ± 0.01^a	0.30 ± 0.04	0.47 ± 0.06
E51A	28.6 ± 4.3 (n = 5)	144.5 ± 9.5 (n = 5)	0.20 ± 0.03	−0.03 ± 0.15	−0.07 ± 0.17

				Linear energy analysis
Mutant	Tau on, msec	Tau recovery, msec	K_eq	ΔΔG(τ_on), kT	ΔΔG(K_eq), kT
WT	29.5 ± 0.16 (n = 17)	138.8 ± 4.9 (n = 27)	0.21 ± 0.01
R18A	98 ± 3.8 (n = 12)^a	123 ± 5.6 (n = 5)^b	0.80 ± 0.05^a	1.20 ± 0.04	1.32 ± 0.07
R18E	401.6 ± 15.7 (n = 16)^a	127.4 ± 5.6 (n = 15)^a	3.15 ± 0.18^a	2.61 ± 0.04	2.70 ± 0.07
D19A	29.6 ± 0.9 (n = 7)	232.4 ± 12 (n = 8)^a	0.13 ± 0.01^a	0.00 ± 0.03	−0.51 ± 0.07
D19K	22.5 ± 0.8 (n = 7)^a	355.5 ± 6.2 (n = 5)^a	0.06 ± 0.003^a	−0.27 ± 0.04	−1.21 ± 0.05
R26A	47.6 ± 3.3 (n = 9)^a	106 ± 8 (n = 9)^a	0.45 ± 0.05^a	0.48 ± 0.07	0.75 ± 0.11
R30A	47.6 ± 2.3 (n = 9)^a	100.7 ± 4.2 (n = 9)^a	0.47 ± 0.03^a	0.48 ± 0.05	0.80 ± 0.07
R38A	42.3 ± 1.3 (n = 11)^a	116 ± 6.5 (n = 8)^a	0.36 ± 0.02^a	0.36 ± 0.03	0.54 ± 0.07
K42A	41.6 ± 2.4 (n = 10)^a	103.9 ± 5.1 (n = 10)^a	0.40 ± 0.03^a	0.34 ± 0.06	0.63 ± 0.08
E45A	26.8 ± 2.6 (n = 4)	106.4 ± 12 (n = 4)	0.25 ± 0.04	−0.10 ± 0.10	0.17 ± 0.15
E46A	21.2 ± 0.6 (n = 8)^a	152.5 ± 5.9 (n = 8)^b	0.14 ± 0.01^a	−0.33 ± 0.03	−0.42 ± 0.06
E46K	31.3 ± 1.3 (n = 7)	175.5 ± 0.8 (n = 7)^a	0.18 ± 0.01^a	0.06 ± 0.04	−0.17 ± 0.05
D47A	40 ± 1.5 (n = 6)^a	117 ± 2.5 (n = 5)^a	0.34 ± 0.01^a	0.30 ± 0.04	0.47 ± 0.06
E51A	28.6 ± 4.3 (n = 5)	144.5 ± 9.5 (n = 5)	0.20 ± 0.03	−0.03 ± 0.15	−0.07 ± 0.17

^aP < 0.01 and ^bP < 0.05 compared to wild type.