TABLE I. AKv1 scanning mutations Linear... | Rockefeller University Press

TABLE I.

AKv1 scanning mutations

				Linear energy analysis
Mutant	Tau on, msec	Tau recovery, msec	K_eq	ΔΔG(τ_on), kT	ΔΔG(K_eq), kT
WT	29.5 ± 0.16 (n = 17)	138.8 ± 4.9 (n = 27)	0.21 ± 0.01
EVA(2-4)ATT	10.7 ± 0.7 (n = 9)^a	12.8 ± 0.7 (n = 3)^a	0.84 ± 0.07^a	−1.01 ± 0.07	1.37 ± 0.09
7-15 Ala (f)	21.4 ± 1.6 (n = 5)^b	1551 ± 103 (n = 3)^a	0.01 ± 0.001^a	−0.32 ± 0.07	−2.73 ± 0.11
7-15 Ala (s)	335.7 ± 21.6 (n = 5)^a	1551 ± 103 (n = 3)^a	0.22 ± 0.02	2.43 ± 0.06	0.02 ± 0.10
16-25 Ala	504.9 ± 30.8 (n = 4)^a	103.7 ± 30.8 (n = 4)^b	4.63 ± 0.51^a	2.84 ± 0.06	3.13 ± 0.31
36-45 Ala	43 ± 2.9 (n = 5)^a	82.7 ± 8.2 (n = 5)^a	0.52 ± 0.06^a	0.38 ± 0.07	0.89 ± 0.13
46-55 Ala	52.3 ± 2 (n = 6)^a	105 ± 4.2 (n = 3)	0.50 ± 0.12^a	0.57 ± 0.04	0.85 ± 0.07

				Linear energy analysis
Mutant	Tau on, msec	Tau recovery, msec	K_eq	ΔΔG(τ_on), kT	ΔΔG(K_eq), kT
WT	29.5 ± 0.16 (n = 17)	138.8 ± 4.9 (n = 27)	0.21 ± 0.01
EVA(2-4)ATT	10.7 ± 0.7 (n = 9)^a	12.8 ± 0.7 (n = 3)^a	0.84 ± 0.07^a	−1.01 ± 0.07	1.37 ± 0.09
7-15 Ala (f)	21.4 ± 1.6 (n = 5)^b	1551 ± 103 (n = 3)^a	0.01 ± 0.001^a	−0.32 ± 0.07	−2.73 ± 0.11
7-15 Ala (s)	335.7 ± 21.6 (n = 5)^a	1551 ± 103 (n = 3)^a	0.22 ± 0.02	2.43 ± 0.06	0.02 ± 0.10
16-25 Ala	504.9 ± 30.8 (n = 4)^a	103.7 ± 30.8 (n = 4)^b	4.63 ± 0.51^a	2.84 ± 0.06	3.13 ± 0.31
36-45 Ala	43 ± 2.9 (n = 5)^a	82.7 ± 8.2 (n = 5)^a	0.52 ± 0.06^a	0.38 ± 0.07	0.89 ± 0.13
46-55 Ala	52.3 ± 2 (n = 6)^a	105 ± 4.2 (n = 3)	0.50 ± 0.12^a	0.57 ± 0.04	0.85 ± 0.07

^aP < 0.01 and ^bP < 0.05 compared to wild type.