TABLE I. Summary of basal bath Δ[HCO 3... | Rockefeller University Press

TABLE I.

Summary of basal bath Δ[HCO₃]-induced pH_i responses

Inhibitor/condition^a		2.62 mM basal bath [HCO₃]-induced pH_i response^b
Apical	Basal	Data	Control	w/inhibitor	Recovery	P^c	n
	DIDS	ΔpH_i	−0.20 ± 0.04	−0.09 ± 0.05	−0.12 ± 0.03	<0.05	5
	DIDS	H⁺ flux	−6.5 ± 1.2	−3.6 ± 2.7	−4.3 ± 1.3	<0.05	5
Na-free	Na-free	ΔpH_i	−0.21 ± 0.04	−0.08 ± 0.01	−0.14 ± 0.02	<0.05	3
Na-free	Na-free	H⁺ flux	−7.2 ± 1.7	−2.8 ± 0.7	−4.9 ± 0.8	<0.05	3
amiloride		ΔpH_i	−0.22 ± 0.03	−0.28 ± 0.05	−0.21 ± 0.04	<0.05	5
amiloride		H⁺ flux	−7.1 ± 1.3	−8.3 ± 1.6	−5.7 ± 1.4	<0.05	5
bumetanide		ΔpH_i	−0.20 ± 0.03	−0.21 ± 0.03	−0.22 ± 0.03	>0.05	4
bumetanide		H⁺ flux	−6.9 ± 1.8	−6.8 ± 0.9	−7.6 ± 1.6	>0.05	4
ouabain		ΔpH_i	−0.18 ± 0.02	−0.20 ± 0.02	−0.23 ± 0.03	>0.05	3
ouabain		H⁺ flux	−5.1 ± 2.1	−5.4 ± 3.2	−5.7 ± 2.7	>0.05	3
DIDS		ΔpH_i	−0.19 ± 0.03	−0.23 ± 0.03	−0.21 ± 0.01	<0.05	7
DIDS		H⁺ flux	−5.0 ± 1.1	−6.0 ± 1.1	−5.3 ± 0.9	<0.05	7
	DZA	ΔpH_i	−0.17 ± 0.01	−0.18 ± 0.03	−0.18 ± 0.02	>0.05	4
	DZA	H⁺ flux	−5.2 ± 0.3	−5.1 ± 0.7	−5.6 ± 0.4	>0.05	4
13% CO₂		ΔpH_i	−0.21 ± 0.05	−0.20 ± 0.03	−0.24 ± 0.04	>0.05	9
13% CO₂		H⁺ flux	−6.0 ± 1.3	−6.1 ± 2.3	−6.5 ± 1.4	>0.05	9
1% CO₂		ΔpH_i	−0.24 ± 0.08	−0.20 ± 0.06	−0.20 ± 0.06	>0.05	5
1% CO₂		H⁺ flux	−7.2 ± 1.8	−10.7 ± 1.9	−6.0 ± 1.3	<0.05	5

Inhibitor/condition^a		2.62 mM basal bath [HCO₃]-induced pH_i response^b
Apical	Basal	Data	Control	w/inhibitor	Recovery	P^c	n
	DIDS	ΔpH_i	−0.20 ± 0.04	−0.09 ± 0.05	−0.12 ± 0.03	<0.05	5
	DIDS	H⁺ flux	−6.5 ± 1.2	−3.6 ± 2.7	−4.3 ± 1.3	<0.05	5
Na-free	Na-free	ΔpH_i	−0.21 ± 0.04	−0.08 ± 0.01	−0.14 ± 0.02	<0.05	3
Na-free	Na-free	H⁺ flux	−7.2 ± 1.7	−2.8 ± 0.7	−4.9 ± 0.8	<0.05	3
amiloride		ΔpH_i	−0.22 ± 0.03	−0.28 ± 0.05	−0.21 ± 0.04	<0.05	5
amiloride		H⁺ flux	−7.1 ± 1.3	−8.3 ± 1.6	−5.7 ± 1.4	<0.05	5
bumetanide		ΔpH_i	−0.20 ± 0.03	−0.21 ± 0.03	−0.22 ± 0.03	>0.05	4
bumetanide		H⁺ flux	−6.9 ± 1.8	−6.8 ± 0.9	−7.6 ± 1.6	>0.05	4
ouabain		ΔpH_i	−0.18 ± 0.02	−0.20 ± 0.02	−0.23 ± 0.03	>0.05	3
ouabain		H⁺ flux	−5.1 ± 2.1	−5.4 ± 3.2	−5.7 ± 2.7	>0.05	3
DIDS		ΔpH_i	−0.19 ± 0.03	−0.23 ± 0.03	−0.21 ± 0.01	<0.05	7
DIDS		H⁺ flux	−5.0 ± 1.1	−6.0 ± 1.1	−5.3 ± 0.9	<0.05	7
	DZA	ΔpH_i	−0.17 ± 0.01	−0.18 ± 0.03	−0.18 ± 0.02	>0.05	4
	DZA	H⁺ flux	−5.2 ± 0.3	−5.1 ± 0.7	−5.6 ± 0.4	>0.05	4
13% CO₂		ΔpH_i	−0.21 ± 0.05	−0.20 ± 0.03	−0.24 ± 0.04	>0.05	9
13% CO₂		H⁺ flux	−6.0 ± 1.3	−6.1 ± 2.3	−6.5 ± 1.4	>0.05	9
1% CO₂		ΔpH_i	−0.24 ± 0.08	−0.20 ± 0.06	−0.20 ± 0.06	>0.05	5
1% CO₂		H⁺ flux	−7.2 ± 1.8	−10.7 ± 1.9	−6.0 ± 1.3	<0.05	5

Blank cells indicate that control Ringer was perfused into the corresponding bath.

H⁺ flux has units of mM × min⁻¹, and all values are reported as mean ± SD.

The basal bath Δ[HCO₃]-induced pH_i response in control Ringer was compared to the pH_i response in the presence of the inhibitor/condition; P < 0.05 is considered significant by Student’s t test.

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