TABLE III Activation and Decay of Ca 2... | Rockefeller University Press

TABLE III

Activation and Decay of Ca²⁺ Transients during Local Depolarizations

	ΔF/F (ΔF/F)	t_peak (s)	t_{1/2 decay} (s)	r₂₀ (%)
normal (2 mM Ca²⁺)	3.25 ± 0.35 (24)	3.76 ± 0.34	10.64 ± 0.98	31.92 ± 5.03
normal (0 mM Ca²⁺)	1.31 ± 0.15^{^a} (12)	1.97 ± 0.22^{^b}	4.98 ± 0.45^{^a}	4.36 ± 2.38^{^a}
normal + 100 μM Gd³⁺(2 mM Ca²⁺)	1.77 ± 0.18^{^b} (14)	1.94 ± 0.13^{^a}	3.49 ± 0.47^{^a}	2.23 ± 0.74^{^a}
dysgenic + SkEIIIK (2 mM Ca²⁺)	0.90 ± 0.18^{^a} (9)	3.51 ± 0.50	4.47 ± 0.52^{^a}	10.41 ± 2.70^{^c}
dysgenic + SkEIIIK (0 mM Ca²⁺)	0.25 ± 0.09^{^a} (5)	2.70 ± 0.45	5.68 ± 1.12^{^c}	4.97 ± 2.17^{^c}
dysgenic (2 mM Ca²⁺)	no response (12)
dyspedic (2 mM Ca²⁺)	no response (14)
β₁ null (2 mM Ca²⁺)	no response (10)
dysgenic + SkEIIIK + ryanodine (2 mM Ca²⁺)	no response (20)

	ΔF/F (ΔF/F)	t_peak (s)	t_{1/2 decay} (s)	r₂₀ (%)
normal (2 mM Ca²⁺)	3.25 ± 0.35 (24)	3.76 ± 0.34	10.64 ± 0.98	31.92 ± 5.03
normal (0 mM Ca²⁺)	1.31 ± 0.15^a (12)	1.97 ± 0.22^b	4.98 ± 0.45^a	4.36 ± 2.38^a
normal + 100 μM Gd³⁺(2 mM Ca²⁺)	1.77 ± 0.18^b (14)	1.94 ± 0.13^a	3.49 ± 0.47^a	2.23 ± 0.74^a
dysgenic + SkEIIIK (2 mM Ca²⁺)	0.90 ± 0.18^a (9)	3.51 ± 0.50	4.47 ± 0.52^a	10.41 ± 2.70^c
dysgenic + SkEIIIK (0 mM Ca²⁺)	0.25 ± 0.09^a (5)	2.70 ± 0.45	5.68 ± 1.12^c	4.97 ± 2.17^c
dysgenic (2 mM Ca²⁺)	no response (12)
dyspedic (2 mM Ca²⁺)	no response (14)
β₁ null (2 mM Ca²⁺)	no response (10)
dysgenic + SkEIIIK + ryanodine (2 mM Ca²⁺)	no response (20)

Data presented here were obtained with depolarizations evoked by 20-s applications of 80 mM KCl Ringer's solution via a micropipette placed near the cell of interest. Where indicated, 100 μM Gd³⁺ was added to the 80 mM KCl Ringer's solution in the micropipette. See Materials and methods for definitions of t_peak, t_{1/2 decay}, and r₂₀. Data are given as mean ± SEM, with the numbers in parentheses indicating the number of myotubes tested. Footnotes indicate significant differences compared to normal myotubes in 2 mM Ca²⁺ by unpaired t test:

P < 0.001.

P < 0.005.

P < 0.05.

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