TABLE I.

Time constants of recovery of sensitivity at the base and tip of the rod outer segment

 n τb τt Tip/Base 
  s s  
No whole cell, 50% bleach 200 ± 30 680 ± 140 3.4 ± 0.29 
Whole cell (0 mM NADPH), 50% bleach 290 ± 20 860 ± 80 3.1 ± 0.29a 
Whole cell (1.66 mM NADPH), 50% bleach 220 ± 30 640 ± 110 3.1 ± 0.45 
Whole cell (5 mM NADPH), 50% bleach 280 ± 50 330 ± 60 1.2 ± 0.04a 
No whole cell, 90% bleach 210 ± 40 — — 
 n τb τt Tip/Base 
  s s  
No whole cell, 50% bleach 200 ± 30 680 ± 140 3.4 ± 0.29 
Whole cell (0 mM NADPH), 50% bleach 290 ± 20 860 ± 80 3.1 ± 0.29a 
Whole cell (1.66 mM NADPH), 50% bleach 220 ± 30 640 ± 110 3.1 ± 0.45 
Whole cell (5 mM NADPH), 50% bleach 280 ± 50 330 ± 60 1.2 ± 0.04a 
No whole cell, 90% bleach 210 ± 40 — — 

The mean time constant at the base of the outer segment (τb) and the tip of the outer segment (τt), and the tip/base time constant ratio are reported. All values are mean ± SEM for a number of cells, n.

a

Student’s t test demonstrates a statistically significant difference between whole cell control solution (0 mM NADPH) and whole cell (5 mM NADPH); P < 0.005.

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