Analyzed Data of the Glycine Scanning Mutagenesis
| Mutants . | Activation . | V1/2 (mV) . | EC50 (μM) . |
|---|---|---|---|
| G344A/S340G | (−) | 20.3 ± 8.0 (n = 6) | |
| G344A/I341G | (+) | −81.2 ± 7.3 (n = 7) | 10.1 ± 1.5 (n = 6) |
| G344A/G342G (G344A) | (−) | 24.6 ± 9.9 (n = 6) | |
| G344A/V343G | (+) | 6.4 ± 8.4 (n = 4) | 48.4 ± 22.0 (n = 5) |
| G344A/A344G (WT) | (+) | −54.2 ± 7.5 (n = 7) | 13.5 ± 2.5 (n = 6) |
| G344A/S345G | (−) | 47.5 ± 10.0 (n = 5) | |
| G344A/F346G | (+) | −105.6 ± 5.0 (n = 6) | 13.3 ± 2.0 (n = 11) |
| G344A/L347G | (+) | <−200 (n = 8) | 24.1 ± 9.3 (n = 6) |
| G344A/C348G | (−) | 51.1 ± 17.8 (n = 5) | |
| G344A/D349G | Nonfunction | Nonfunction | Nonfunctional |
| G344A/W350G | (−) | 28.2 ± 7.8 (n = 5) |
| Mutants . | Activation . | V1/2 (mV) . | EC50 (μM) . |
|---|---|---|---|
| G344A/S340G | (−) | 20.3 ± 8.0 (n = 6) | |
| G344A/I341G | (+) | −81.2 ± 7.3 (n = 7) | 10.1 ± 1.5 (n = 6) |
| G344A/G342G (G344A) | (−) | 24.6 ± 9.9 (n = 6) | |
| G344A/V343G | (+) | 6.4 ± 8.4 (n = 4) | 48.4 ± 22.0 (n = 5) |
| G344A/A344G (WT) | (+) | −54.2 ± 7.5 (n = 7) | 13.5 ± 2.5 (n = 6) |
| G344A/S345G | (−) | 47.5 ± 10.0 (n = 5) | |
| G344A/F346G | (+) | −105.6 ± 5.0 (n = 6) | 13.3 ± 2.0 (n = 11) |
| G344A/L347G | (+) | <−200 (n = 8) | 24.1 ± 9.3 (n = 6) |
| G344A/C348G | (−) | 51.1 ± 17.8 (n = 5) | |
| G344A/D349G | Nonfunction | Nonfunction | Nonfunctional |
| G344A/W350G | (−) | 28.2 ± 7.8 (n = 5) |
Names of the analyzed mutants are shown in the first column. In the second column, (+) indicates recovery of the activation phase; (−) indicates no recovery. For the mutants with recovered activation phase, tail current analyses were performed, and the V1/2 values in the presence of 300 μM ATP (except 100 μM for G344A/V343G) are shown in the third column. V1/2 values for the G344A/L347G mutant were too hyperpolarized to analyze precisely. EC50 values for the [ATP] response relationship at −160 mV are indicated in the last column. G344A/D349G showed no detectable current.