Boltzmann Fit Parameters for mSlo1 GK-V Relations
| Segments | Channel | V0.5 (mV) | ZAPP (e) | n | [Ca2+]i |
|---|---|---|---|---|---|
| WT | 206.50 ± 3.81 | 1.01 ± 0.050 | 8 | 0 | |
| Native Cys and His | C14A/C277A | 234.18 ± 3.55 | 0.96 ± 0.028 | 6 | 0 |
| C141A/C277A | 230.22 ± 2.97 | 0.94 ± 0.011 | 7 | 0 | |
| C14A/C141C/C277A | 237.91 ± 3.53 | 0.95 ± 0.049 | 7 | 0 | |
| H254A | 169.08 ± 3.23 | 1.24 ± 0.044 | 7 | 5 | |
| S1 | Y130A | 202.02 ± 4.31 | 0.64 ± 0.022 | 8 | 0 |
| Y130H | 216.66 ± 3.37 | 0.85 ± 0.059 | 6 | 0 | |
| D133A | 229.72 ± 2.31 | 1.01 ± 0.023 | 19 | 0 | |
| D133C | 238.21 ± 1.48 | 1.08 ± 0.023 | 17 | 0 | |
| D133H | 224.15 ± 1.56 | 1.04 ± 0.026 | 14 | 0 | |
| S2 | T149C | 202.47 ± 2.48 | 0.93 ± 0.029 | 5 | 0 |
| Q151A | 230.56 ± 3.01 | 0.96 ± 0.030 | 13 | 0 | |
| Q151C | 230.56 ± 2.72 | 0.99 ± 0.022 | 18 | 0 | |
| Q151H | 229.60 ± 3.71 | 0.98 ± 0.029 | 12 | 0 | |
| D153K | 201.96 ± 3.22 | 0.84 ± 0.030 | 23 | 50 | |
| D153H | 207.79 ± 3.99 | 0.84 ± 0.034 | 6 | 50 | |
| M154A | 200.39 ± 3.10 | 0.96 ± 0.027 | 7 | 0 | |
| M154C | 201.62 ± 4.62 | 0.84 ± 0.023 | 5 | 0 | |
| N158A | 197.61 ± 2.89 | 0.81 ± 0.015 | 4 | 0 | |
| N158C | 196.03 ± 4.18 | 0.83 ± 0.019 | 4 | 0 | |
| S3 | D186A | 148.92 ± 2.98 | 0.95 ± 0.051 | 5 | 1 |
| D186H | 144.55 ± 2.58 | 0.97 ± 0.032 | 6 | 1 | |
| T189A | 232.94 ± 5.49 | 0.95 ± 0.026 | 4 | 0 | |
| T189C | 231.51 ± 2.58 | 1.10 ± 0.047 | 3 | 0 | |
| S196A | 212.36 ± 1.77 | 0.95 ± 0.026 | 5 | 0 | |
| S196C | 213.70 ± 2.67 | 0.98 ± 0.030 | 4 | 0 | |
| Y198A | 213.05 ± 4.32 | 0.83 ± 0.017 | 6 | 0 | |
| Y198C | 216.88 ± 3.10 | 0.89 ± 0.030 | 7 | 0 | |
| S4 | R207Q | 149.66 ± 2.34 | 0.62 ± 0.016 | 8 | 0 |
| R207C | 147.85 ± 2.90 | 0.61 ± 0.017 | 10 | 0 | |
| R210N | 158.12 ± 6.96 | 0.60 ± 0.019 | 6 | 1 | |
| R210C | 158.47 ± 5.97 | 0.55 ± 0.028 | 9 | 0 |
| Segments | Channel | V0.5 (mV) | ZAPP ( | [Ca2+]i | |
|---|---|---|---|---|---|
| WT | 206.50 ± 3.81 | 1.01 ± 0.050 | 8 | 0 | |
| Native Cys and His | C14A/C277A | 234.18 ± 3.55 | 0.96 ± 0.028 | 6 | 0 |
| C141A/C277A | 230.22 ± 2.97 | 0.94 ± 0.011 | 7 | 0 | |
| C14A/C141C/C277A | 237.91 ± 3.53 | 0.95 ± 0.049 | 7 | 0 | |
| H254A | 169.08 ± 3.23 | 1.24 ± 0.044 | 7 | 5 | |
| Y130A | 202.02 ± 4.31 | 0.64 ± 0.022 | 8 | 0 | |
| Y130H | 216.66 ± 3.37 | 0.85 ± 0.059 | 6 | 0 | |
| D133A | 229.72 ± 2.31 | 1.01 ± 0.023 | 19 | 0 | |
| D133C | 238.21 ± 1.48 | 1.08 ± 0.023 | 17 | 0 | |
| D133H | 224.15 ± 1.56 | 1.04 ± 0.026 | 14 | 0 | |
| T149C | 202.47 ± 2.48 | 0.93 ± 0.029 | 5 | 0 | |
| Q151A | 230.56 ± 3.01 | 0.96 ± 0.030 | 13 | 0 | |
| Q151C | 230.56 ± 2.72 | 0.99 ± 0.022 | 18 | 0 | |
| Q151H | 229.60 ± 3.71 | 0.98 ± 0.029 | 12 | 0 | |
| D153K | 201.96 ± 3.22 | 0.84 ± 0.030 | 23 | 50 | |
| D153H | 207.79 ± 3.99 | 0.84 ± 0.034 | 6 | 50 | |
| M154A | 200.39 ± 3.10 | 0.96 ± 0.027 | 7 | 0 | |
| M154C | 201.62 ± 4.62 | 0.84 ± 0.023 | 5 | 0 | |
| N158A | 197.61 ± 2.89 | 0.81 ± 0.015 | 4 | 0 | |
| N158C | 196.03 ± 4.18 | 0.83 ± 0.019 | 4 | 0 | |
| D186A | 148.92 ± 2.98 | 0.95 ± 0.051 | 5 | 1 | |
| D186H | 144.55 ± 2.58 | 0.97 ± 0.032 | 6 | 1 | |
| T189A | 232.94 ± 5.49 | 0.95 ± 0.026 | 4 | 0 | |
| T189C | 231.51 ± 2.58 | 1.10 ± 0.047 | 3 | 0 | |
| S196A | 212.36 ± 1.77 | 0.95 ± 0.026 | 5 | 0 | |
| S196C | 213.70 ± 2.67 | 0.98 ± 0.030 | 4 | 0 | |
| Y198A | 213.05 ± 4.32 | 0.83 ± 0.017 | 6 | 0 | |
| Y198C | 216.88 ± 3.10 | 0.89 ± 0.030 | 7 | 0 | |
| R207Q | 149.66 ± 2.34 | 0.62 ± 0.016 | 8 | 0 | |
| R207C | 147.85 ± 2.90 | 0.61 ± 0.017 | 10 | 0 | |
| R210N | 158.12 ± 6.96 | 0.60 ± 0.019 | 6 | 1 | |
| R210C | 158.47 ± 5.97 | 0.55 ± 0.028 | 9 | 0 |
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