TABLE I

Summary of Kinetic Parameters for QA Block of TRPV1a

Blocker
Association rate constant kon(0 mV), (M−1s−1)
Dissociation rate constant koff (0 mV), (s−1)
zon (eo)
zoff (eo)
TEA (n = 3) 1.04 × 106 ± 4.8 × 105 2.89 × 104 ± 3.2 × 103 0.94 ± 0.13 0.27 ± 0.03 
TPrA (n = 3) 8.59 × 105 ± 1.5 × 105 1.23 × 104 ± 186 0.69 ± 0.14 −0.19 ± 0.01 
TBA (n = 6) 2.5 × 105 ± 2.9 × 104 83.76 ± 10 0.93 ± 0.03 −0.63 ± 0.03 
TPA (n = 5) 1.03 × 105 ± 4 × 104 10.73 ± 0.95 0.68 ± 0.06 −0.45 ± 0.02 
Blocker
Association rate constant kon(0 mV), (M−1s−1)
Dissociation rate constant koff (0 mV), (s−1)
zon (eo)
zoff (eo)
TEA (n = 3) 1.04 × 106 ± 4.8 × 105 2.89 × 104 ± 3.2 × 103 0.94 ± 0.13 0.27 ± 0.03 
TPrA (n = 3) 8.59 × 105 ± 1.5 × 105 1.23 × 104 ± 186 0.69 ± 0.14 −0.19 ± 0.01 
TBA (n = 6) 2.5 × 105 ± 2.9 × 104 83.76 ± 10 0.93 ± 0.03 −0.63 ± 0.03 
TPA (n = 5) 1.03 × 105 ± 4 × 104 10.73 ± 0.95 0.68 ± 0.06 −0.45 ± 0.02 

The table summarizes the on- and off-rates for the four blockers used in this study and their associated valence.

a

Data are mean ± SEM.

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