Properties of Ca2+ Sparks and STICs
. | SM (Ca2+ ions)b . | ICa(spark) . | ΔF/F0 (%)c . |
|---|---|---|---|
| (A) Ca2+ sparksa | |||
| Amplitude | 276,000 ± 46,000 (29)e | 8.5 ± 1.2f (29) | 46 ± 5 (23) |
| 235,000 ± 34,500 (159) | 4.1 ± 0.5g (159) | ||
| Duration (ms)d | 44 ± 5 (23) | 162 ± 13 (23) | |
| Rise Time (ms) | 9 ± 2 (29) | 24 ± 2 (23) | |
| Taudecay (ms) | 12 ± 2 (23) | 55 ± 5 (23) | |
| Amplitude (pA) | Taurise (ms) | Taudecay (ms) | |
| (B) STICsa | 35 ± 5 (138) | 21 ± 3 (97) | 98 ± 8 (77) |
. | SM (Ca2+ ions)b . | ICa(spark) . | ΔF/F0 (%)c . |
|---|---|---|---|
| (A) Ca2+ sparksa | |||
| Amplitude | 276,000 ± 46,000 (29)e | 8.5 ± 1.2f (29) | 46 ± 5 (23) |
| 235,000 ± 34,500 (159) | 4.1 ± 0.5g (159) | ||
| Duration (ms)d | 44 ± 5 (23) | 162 ± 13 (23) | |
| Rise Time (ms) | 9 ± 2 (29) | 24 ± 2 (23) | |
| Taudecay (ms) | 12 ± 2 (23) | 55 ± 5 (23) | |
| Amplitude (pA) | Taurise (ms) | Taudecay (ms) | |
| (B) STICsa | 35 ± 5 (138) | 21 ± 3 (97) | 98 ± 8 (77) |
Recorded simultaneously at −80 mV.
Corrected for endogenous fixed Ca2+ buffer.
Fluorescence change in the epicenter pixel of the sparks.
Time between the onset of the event to 92% of the decay (i.e., 2.5 times of decay Tau).
Number in parentheses depicts the number of events.
Measured by time differentiation of SM; unit, pA.
Estimated by fitting 10–90% of SM rise phase; unit, pA.