14C-sucrose Space in Resting and Stimulated Soleus and EDL Muscles
. | 14C-sucrose space (% of wet wt.) . |
|---|---|
| Resting soleus | 22.9 ± 3.4 |
| Soleus stimulated for 60 s | 24.2 ± 2.8 |
| Resting EDL | 21.7 ± 1.9 |
| EDL stimulated for 30 s | 20.9 ± 1.2 |
. | 14C-sucrose space (% of wet wt.) . |
|---|---|
| Resting soleus | 22.9 ± 3.4 |
| Soleus stimulated for 60 s | 24.2 ± 2.8 |
| Resting EDL | 21.7 ± 1.9 |
| EDL stimulated for 30 s | 20.9 ± 1.2 |
Muscles were mounted on electrodes for isometric contractions and incubated for 90 min in K.R. containing 1 mM sucrose and 3.7 kBq/ml of 14C-sucrose. Then all muscles were moved into empty tubes and carefully blotted to remove the last drips of buffer and stimulated at 60 Hz or allowed to rest. Immediately after the stimulation, the muscles were blotted, weighed, and soaked overnight in 0.3 M TCA. The TCA extract was sampled for counting of 14C-sucrose. Each value is the mean of measurements on 3 muscles ± SEM.