Effect of CaM and CaM1234 Overexpression on NaV1.4, NaV1.4-EYFP and NaV1.41740-EYFP
| Channel/Mutant . | V1/2 steady state inactivation . | Decay time constant τh . | V1/2 activation . | Current density . |
|---|---|---|---|---|
| mV | ms at −20 mV | mV | pA/pF | |
| NaV1.4-EYFP + β1 | −65.2 ± 0.4 (n = 5) | 0.69 ± 0.04 | −32.2 ± 0.1 | −426.4 ± 93.3 |
| NaV1.4-EYFP + ECFP-CaM + β1 | −71 ± 0.2 (n = 5) | 0.77 ± 0.05 | −32.6 ± 0.2 | −458.0 ± 54.0 |
| NaV1.4-EYFP + ECFP-CaM1234 + β1 | −64.2 ± 0.1 (n = 5) | 0.66 ± 0.02 | −32.9 ± 0.1 | −269.6 ± 55.5 |
| NaV1.4 + β1 | −65.08 ± 0.1 (n = 6) | 0.61 ± 0.07 | −32.1 ± 0.3 | −453.2 ± 71.5 |
| NaV1.4 + ECFP-CaM + β1 | −69.3 ± 0.1 (n = 7) | 0.63 ± 0.02 | −30.6 ± 0.2 | −309.4 ± 112.0 |
| NaV1.4 + ECFP-CaM1234 + β1 | −64.3 ± 0.3 (n = 7) | 0.66 ± 0.07 | −31.2 ± 0.1 | −324.1 ± 80.9 |
| NaV1.4IQ/AA + β1 | −79.4 ± 0.2 (n = 6) | 1.3 ± 0.2 | −40.9 ± 0.3 | −307.8 ± 89.9 |
| NaV1.4IQ/AA + ECFP-CaM + β1 | −80.0 ± 0.4 (n = 6) | 1.25 ± 0.15 | −40.0 ± 0.1 | −242.5 ± 60.5 |
| NaV1.4IQ/AA + ECFP-CaM1234 + β1 | −80.7 ± 0.3 (n =5) | 1.12 ± 0.15 | −41.0 ± 0.3 | −254.2 ± 73.1 |
| NaV1.41740 + β1 | −62.7 ± 0.1 (n = 6) | 0.69 ± 0.09 | −30.1 ± 0.2 | −437.8 ± 116.0 |
| NaV1.41740 + ECFP-CaM + β1 | −68.0 ± 0.1 (n = 8) | 0.62 ± 0.02 | −29.5 ± 0.1 | −294.0 ± 74.7 |
| NaV1.41740 + ECFP-CaM1234 + β1 | −64.0 ± 0.04 (n = 5) | 0.79 ± 0.01 | −31.1 ± 0.2 | −295.0 ± 60.9 |
| NaV1.41740-G4-CaM + β1 | −65.8 ± 0.1 (n = 8) | 0.69 ± 0.09 | −30.8 ± 0.3 | −372.9 ± 67.4 |
| NaV1.41740-G4-CaM + ECFP-CaM + β1 | −71.4 ± 0.2 (n = 9) | 0.55 ± 0.3 | −30.7 ± 0.1 | −373.0 ± 77.5 |
| NaV1.41740-G4-CaM + ECFP-CaM1234 + β1 | −63.6 ± 0.2 (n = 5) | 0.52 ± 0.1 | −32.1 ± 0.2 | −413.5 ± 118.9 |
| NaV1.41740-G14-CaM + β1 | −68.4 ± 0.2 (n = 9) | 0.60 ± 0.1 | −31.9 ± 0.3 | −201.4 ± 63.2 |
| NaV1.41740-G14-CaM + ECFP-CaM + β1 | −67.7 ± 0.2 (n = 9) | 0.51 ± 0.06 | −30.2 ± 0.3 | −391.5 ± 121.1 |
| NaV1.41740-G14-CaM + ECFP-CaM1234 + β1 | −66.7 + 0.1 (n = 9) | 0.53 ± 0.06 | −32.0 ± 0.1 | −439.5 ± 141.9 |
| Channel/Mutant . | V1/2 steady state inactivation . | Decay time constant τh . | V1/2 activation . | Current density . |
|---|---|---|---|---|
| mV | ms at −20 mV | mV | pA/pF | |
| NaV1.4-EYFP + β1 | −65.2 ± 0.4 (n = 5) | 0.69 ± 0.04 | −32.2 ± 0.1 | −426.4 ± 93.3 |
| NaV1.4-EYFP + ECFP-CaM + β1 | −71 ± 0.2 (n = 5) | 0.77 ± 0.05 | −32.6 ± 0.2 | −458.0 ± 54.0 |
| NaV1.4-EYFP + ECFP-CaM1234 + β1 | −64.2 ± 0.1 (n = 5) | 0.66 ± 0.02 | −32.9 ± 0.1 | −269.6 ± 55.5 |
| NaV1.4 + β1 | −65.08 ± 0.1 (n = 6) | 0.61 ± 0.07 | −32.1 ± 0.3 | −453.2 ± 71.5 |
| NaV1.4 + ECFP-CaM + β1 | −69.3 ± 0.1 (n = 7) | 0.63 ± 0.02 | −30.6 ± 0.2 | −309.4 ± 112.0 |
| NaV1.4 + ECFP-CaM1234 + β1 | −64.3 ± 0.3 (n = 7) | 0.66 ± 0.07 | −31.2 ± 0.1 | −324.1 ± 80.9 |
| NaV1.4IQ/AA + β1 | −79.4 ± 0.2 (n = 6) | 1.3 ± 0.2 | −40.9 ± 0.3 | −307.8 ± 89.9 |
| NaV1.4IQ/AA + ECFP-CaM + β1 | −80.0 ± 0.4 (n = 6) | 1.25 ± 0.15 | −40.0 ± 0.1 | −242.5 ± 60.5 |
| NaV1.4IQ/AA + ECFP-CaM1234 + β1 | −80.7 ± 0.3 (n =5) | 1.12 ± 0.15 | −41.0 ± 0.3 | −254.2 ± 73.1 |
| NaV1.41740 + β1 | −62.7 ± 0.1 (n = 6) | 0.69 ± 0.09 | −30.1 ± 0.2 | −437.8 ± 116.0 |
| NaV1.41740 + ECFP-CaM + β1 | −68.0 ± 0.1 (n = 8) | 0.62 ± 0.02 | −29.5 ± 0.1 | −294.0 ± 74.7 |
| NaV1.41740 + ECFP-CaM1234 + β1 | −64.0 ± 0.04 (n = 5) | 0.79 ± 0.01 | −31.1 ± 0.2 | −295.0 ± 60.9 |
| NaV1.41740-G4-CaM + β1 | −65.8 ± 0.1 (n = 8) | 0.69 ± 0.09 | −30.8 ± 0.3 | −372.9 ± 67.4 |
| NaV1.41740-G4-CaM + ECFP-CaM + β1 | −71.4 ± 0.2 (n = 9) | 0.55 ± 0.3 | −30.7 ± 0.1 | −373.0 ± 77.5 |
| NaV1.41740-G4-CaM + ECFP-CaM1234 + β1 | −63.6 ± 0.2 (n = 5) | 0.52 ± 0.1 | −32.1 ± 0.2 | −413.5 ± 118.9 |
| NaV1.41740-G14-CaM + β1 | −68.4 ± 0.2 (n = 9) | 0.60 ± 0.1 | −31.9 ± 0.3 | −201.4 ± 63.2 |
| NaV1.41740-G14-CaM + ECFP-CaM + β1 | −67.7 ± 0.2 (n = 9) | 0.51 ± 0.06 | −30.2 ± 0.3 | −391.5 ± 121.1 |
| NaV1.41740-G14-CaM + ECFP-CaM1234 + β1 | −66.7 + 0.1 (n = 9) | 0.53 ± 0.06 | −32.0 ± 0.1 | −439.5 ± 141.9 |