Composition of Intracellular solutions
| CsCl | CsF | KCl | EGTA | CaCl2 | NaCl | MgCl2 | Na2GTP | MgATP | HEPES | Figs. | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Solution 1 | 100 | 30 | 0 | 10 | 1 | 8 | 1 | 0.4 | 4 | 10 | 2 (A and B) and 3 |
| Solution 2 | 0 | 30 | 100 | 10 | 1 | 8 | 1 | 0.4 | 4 | 10 | 1 and 4 |
| Solution 3 | 130 | 0 | 0 | 10 | 1 | 8 | 1 | 0.4 | 4 | 10 | 5 and 6 |
| Solution 4 | 30 | 0 | 100 | 10 | 1 | 8 | 1 | 0.4 | 4 | 10 | 7 |
| Solution 5 | 0 | 0 | 130 | 10 | 1 | 8 | 1 | 0.4 | 4 | 10 | 8 |
| CsCl | CsF | KCl | EGTA | CaCl2 | NaCl | MgCl2 | Na2GTP | MgATP | HEPES | Figs. | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Solution 1 | 100 | 30 | 0 | 10 | 1 | 8 | 1 | 0.4 | 4 | 10 | 2 (A and B) and 3 |
| Solution 2 | 0 | 30 | 100 | 10 | 1 | 8 | 1 | 0.4 | 4 | 10 | 1 and 4 |
| Solution 3 | 130 | 0 | 0 | 10 | 1 | 8 | 1 | 0.4 | 4 | 10 | 5 and 6 |
| Solution 4 | 30 | 0 | 100 | 10 | 1 | 8 | 1 | 0.4 | 4 | 10 | 7 |
| Solution 5 | 0 | 0 | 130 | 10 | 1 | 8 | 1 | 0.4 | 4 | 10 | 8 |
Concentrations are expressed in mM. pH adjusted to 7.3 with NaOH, 300 mOsm/liter.
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