Kinetic parameters of the IDH1 R132C mutant enzyme
| Oxidative (→NADPH) | WT | R132C |
| KM,NADP+ (µM) | 49 | 21 |
| KM,isocitrate (µM) | 57 | 8.7 × 104 |
| KM,MgCl2 (µM) | 29 | 4.5 × 102 |
| Ki,αKG (µM) | 6.1 × 102 | 61 |
| kcat (s−1) | 1.3 × 105 | 7.1 × 102 |
| kcat /KM,isoc(M−1.s−1) | 2.3 × 109 | 8.2 × 103 |
| Reductive (→ NADP+) | WT | R132C |
| KM,NADPH (µM) | na | 0.3 |
| KM,αKG (µM) | na | 295 |
| kcat (s−1) | ∼7 | 5.5 × 102 |
| Oxidative (→NADPH) | WT | R132C |
| KM,NADP+ (µM) | 49 | 21 |
| KM,isocitrate (µM) | 57 | 8.7 × 104 |
| KM,MgCl2 (µM) | 29 | 4.5 × 102 |
| Ki,αKG (µM) | 6.1 × 102 | 61 |
| kcat (s−1) | 1.3 × 105 | 7.1 × 102 |
| kcat /KM,isoc(M−1.s−1) | 2.3 × 109 | 8.2 × 103 |
| Reductive (→ NADP+) | WT | R132C |
| KM,NADPH (µM) | na | 0.3 |
| KM,αKG (µM) | na | 295 |
| kcat (s−1) | ∼7 | 5.5 × 102 |
Na indicates no measureable activity.