Table I.

Brief administration of R-Spo1 had no effects on immunophenotype

Immunophenotype +Control +R-Spo1 
Spleen   
CD4+ 20.7 ± 4.2 17.4 ± 2.0 
CD8+ 14.3 ± 2.5 11.9 ± 0.9 
CD4+Foxp3+ 2.46 ± 0.63 2.46 ± 0.34 
B220+ 58.5 ± 10.9 57.8 ± 2.9 
Mesenteric lymph node   
CD4+ 4.3 ± 0.7 4.9 ± 0.2 
CD8+ 3.5 ± 0.7 4.2 ± 0.4 
CD4+Foxp3+ 0.65 ± 0.14 0.70 ± 0.09 
Thymus   
CD4+CD8+ 96.2 ± 16.9 91.6 ± 19.8 
CD4+ 8.4 ± 1.0 8.6 ± 2.1 
CD8+ 3.4 ± 0.3 3.6 ± 0.6 
Immunophenotype +Control +R-Spo1 
Spleen   
CD4+ 20.7 ± 4.2 17.4 ± 2.0 
CD8+ 14.3 ± 2.5 11.9 ± 0.9 
CD4+Foxp3+ 2.46 ± 0.63 2.46 ± 0.34 
B220+ 58.5 ± 10.9 57.8 ± 2.9 
Mesenteric lymph node   
CD4+ 4.3 ± 0.7 4.9 ± 0.2 
CD8+ 3.5 ± 0.7 4.2 ± 0.4 
CD4+Foxp3+ 0.65 ± 0.14 0.70 ± 0.09 
Thymus   
CD4+CD8+ 96.2 ± 16.9 91.6 ± 19.8 
CD4+ 8.4 ± 1.0 8.6 ± 2.1 
CD8+ 3.4 ± 0.3 3.6 ± 0.6 

B6 mice were intravenously injected with R-Spo1 (200 µg/day) or control for 3 d, and the spleen, mesenteric lymph nodes, and thymus were harvested 6 h later. Cell numbers are shown as mean ± SD (×106). Data are representative of two independent experiments (n = 4 for +control and +R-Spo1).

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