Binding constants for the interaction of the DM1 TCR with HLA-B*4402/3/5 (determined by SPR)
| Immobilized ligand | Analyte | Kd (µM) | Kon (×104/Ms) | Koff (1/s) | Kd calc (µM) |
| HLA-B*4402EENL | DM1 TCR | 6.3 ± 0.3 | 1.4 | 0.2 | 14 |
| HLA-B*4403EENL | DM1 TCR | 9.4 ± 0.2 | 1.3 | 0.25 | 16 |
| HLA-B*4405EENL | DM1 TCR | 0.3 ± 0.1 | 6.8 | 0.02 | 0.23 |
| aDM1 TCR | HLA-B*4405EENL | 1 ± 0.5 | ND | ND | ND |
| Immobilized ligand | Analyte | Kd (µM) | Kon (×104/Ms) | Koff (1/s) | Kd calc (µM) |
| HLA-B*4402EENL | DM1 TCR | 6.3 ± 0.3 | 1.4 | 0.2 | 14 |
| HLA-B*4403EENL | DM1 TCR | 9.4 ± 0.2 | 1.3 | 0.25 | 16 |
| HLA-B*4405EENL | DM1 TCR | 0.3 ± 0.1 | 6.8 | 0.02 | 0.23 |
| aDM1 TCR | HLA-B*4405EENL | 1 ± 0.5 | ND | ND | ND |
ND, not determined.
For cross-validation, the affinity for the DM1 TCR–HLA-B*4405EENL interaction was determined. This was similar irrespective of whether the DM1 TCR or the pMHC was coupled to the chip (note that the levels for the HLA-B*4402EENL and HLA-B*4403EENL refolds were too low to permit these MHCs to be the analytes).