The MZ can replenish N− H-CDR3 from adult BM precursors
| Productive | Nonproductive | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Total H-CDR3 sequences | Number of N− H-CDR3 sequences | Percentage N− | Total H-CDR3 sequences | Number of N− H- CDR3 sequences | Percentage N− | |||||
| MZ | 73 | 6 | 8 | 27 | 2 | 7 | ||||
| FO | 43 | 0 | 0 | 17 | 0 | 0 | ||||
| Productive | Nonproductive | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Total H-CDR3 sequences | Number of N− H-CDR3 sequences | Percentage N− | Total H-CDR3 sequences | Number of N− H- CDR3 sequences | Percentage N− | |||||
| MZ | 73 | 6 | 8 | 27 | 2 | 7 | ||||
| FO | 43 | 0 | 0 | 17 | 0 | 0 | ||||
Recipient mice (n = 2) were lethally irradiated, as described in Materials and methods, and reconstituted with B220-depleted BM precursors from littermate donors. After 5 wk of reconstitution, MZ and FO B cells were sorted as previously described. MZ and FO DNA was PCR amplified (four to eight independent PCRs), cloned, and sequenced, and the N− H-CDR3 were analyzed.
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