The MZ can replenish N− H-CDR3 from adult BM precursors
| . | Productive . | . | . | Nonproductive . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
. | Total H-CDR3 sequences . | Number of N− H-CDR3 sequences . | Percentage N− . | Total H-CDR3 sequences . | Number of N− H- CDR3 sequences . | Percentage N− . | ||||
| MZ | 73 | 6 | 8 | 27 | 2 | 7 | ||||
| FO | 43 | 0 | 0 | 17 | 0 | 0 | ||||
| . | Productive . | . | . | Nonproductive . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
. | Total H-CDR3 sequences . | Number of N− H-CDR3 sequences . | Percentage N− . | Total H-CDR3 sequences . | Number of N− H- CDR3 sequences . | Percentage N− . | ||||
| MZ | 73 | 6 | 8 | 27 | 2 | 7 | ||||
| FO | 43 | 0 | 0 | 17 | 0 | 0 | ||||
Recipient mice (n = 2) were lethally irradiated, as described in Materials and methods, and reconstituted with B220-depleted BM precursors from littermate donors. After 5 wk of reconstitution, MZ and FO B cells were sorted as previously described. MZ and FO DNA was PCR amplified (four to eight independent PCRs), cloned, and sequenced, and the N− H-CDR3 were analyzed.