Table II. PAD incubation of CXC and CC... | Rockefeller University Press

Table II.

PAD incubation of CXC and CC chemokines

Chemokine	NH₂-terminal sequence^{^a}	Enzyme	E/S^{^b}	Incubation time^{^c}	Percentage first Cit^{^d}	Percentage second Cit^{^d}	M_r shift^{^e}
CXCL5	AGPAAAVLRELRCVC-	rabbit PAD	1/20	90	89.8	0	+ 1.0
		hu PAD2	1/200	120	14.0	0	+ 0.4
		hu PAD4	1/200	120	35.1	0	+ 0.6
CXCL8	AVLPRSAKELRCQC-	rabbit PAD	1/20	90	100	0	+ 1.1
		hu PAD2	1/200	120	88.0	0	ND
		hu PAD4	1/200	120	94.5	0	+ 1.0
CCL17	ARGTNVGRECC-	hu PAD2	1/200	120	24.8	22.6	+ 0.8
		hu PAD4	1/200	120	92.1	54.3	+ 2.4
CCL26	TRGSDISKTCC-	hu PAD	1/200	120	17.1	ND	+ 0.2
		hu PAD4	1/200	120	17.8	ND	+ 0.2
		hu PAD4	1/40	120	100	ND	+ 1.3

Chemokine	NH₂-terminal sequence^a	Enzyme	E/S^b	Incubation time^c	Percentage first Cit^d	Percentage second Cit^d	M_r shift^e
CXCL5	AGPAAAVLRELRCVC-	rabbit PAD	1/20	90	89.8	0	+ 1.0
		hu PAD2	1/200	120	14.0	0	+ 0.4
		hu PAD4	1/200	120	35.1	0	+ 0.6
CXCL8	AVLPRSAKELRCQC-	rabbit PAD	1/20	90	100	0	+ 1.1
		hu PAD2	1/200	120	88.0	0	ND
		hu PAD4	1/200	120	94.5	0	+ 1.0
CCL17	ARGTNVGRECC-	hu PAD2	1/200	120	24.8	22.6	+ 0.8
		hu PAD4	1/200	120	92.1	54.3	+ 2.4
CCL26	TRGSDISKTCC-	hu PAD	1/200	120	17.1	ND	+ 0.2
		hu PAD4	1/200	120	17.8	ND	+ 0.2
		hu PAD4	1/40	120	100	ND	+ 1.3

NH₂-terminal Arg residues are indicated in bold, cysteine motifs are underlined.

Molar Enzyme/Substrate ratio.

Incubation time in minutes.

Percentages of conversion of NH₂-terminal Arg residues to Cit were determined by Edman degradation.

Shift compared to the theoretical average M_r of the uncitrullinated chemokine as determined by ion trap mass spectrometry.

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