Table I. Identification of natural CXCL8... | Rockefeller University Press

Table I.

Identification of natural CXCL8 by Edman degradation and mass spectrometry

CXCL8 isoform	NH₂-terminal sequence^{^a}
	−2		1				5					10
CXCL8(-2–77)	E	G	A	V	L	P	R	S	A	K	E	L	R	X	Q	X	7.8
CXCL8(-2–77)Cit₅	E	G	A	V	L	P	B	S	A	K	E	L	R	X	Q	X	0.7
CXCL8(1–77)			A	V	L	P	R	S	A	K	E	L	R	X	Q	X	25.9
CXCL8(1–77)Cit₅			A	V	L	P	B	S	A	K	E	L	R	X	Q	X	4.1
CXCL8(2–77)				V	L	P	R	S	A	K	E	L	R	X	Q	X	5.5
CXCL8(2–77)Cit₅				V	L	P	B	S	A	K	E	L	R	X	Q	X	0.7
CXCL8(3–77)					L	P	R	S	A	K	E	L	R	X	Q	X	1.1
CXCL8(6–77)								S	A	K	E	L	R	X	Q	X	44.1
CXCL8(8–77)										K	E	L	R	X	Q	X	9.0
CXCL8(9–77)											E	L	R	X	Q	X	1.2

CXCL8 isoform	NH₂-terminal sequence^{^a}
	−2		1				5					10
CXCL8(-2–77)	E	G	A	V	L	P	R	S	A	K	E	L	R	X	Q	X	7.8
CXCL8(-2–77)Cit₅	E	G	A	V	L	P	B	S	A	K	E	L	R	X	Q	X	0.7
CXCL8(1–77)			A	V	L	P	R	S	A	K	E	L	R	X	Q	X	25.9
CXCL8(1–77)Cit₅			A	V	L	P	B	S	A	K	E	L	R	X	Q	X	4.1
CXCL8(2–77)				V	L	P	R	S	A	K	E	L	R	X	Q	X	5.5
CXCL8(2–77)Cit₅				V	L	P	B	S	A	K	E	L	R	X	Q	X	0.7
CXCL8(3–77)					L	P	R	S	A	K	E	L	R	X	Q	X	1.1
CXCL8(6–77)								S	A	K	E	L	R	X	Q	X	44.1
CXCL8(8–77)										K	E	L	R	X	Q	X	9.0
CXCL8(9–77)											E	L	R	X	Q	X	1.2

X stands for an unidentified residue (on Cys positions) and B stands for citrulline. Numbering corresponds to the position of the amino acids in CXCL8(1-77).

The relative amount of the different CXCL8 isoforms was obtained from the yields for the different amino acids during Edman degradation on >20 RP-HPLC fractions from a large batch of leukocytes from pooled blood donations (Table S1).

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