Table I.

Summary of measured mitotic parameters

Experiment Metaphase flux velocity Anaphase kMT minus-end depolymerization velocity Inter-KT distance measured from fixed cells Pixel intensity BubR1/CID Inter-KT distance measured from live imaging Chromosome poleward velocity [Vch] Half-spindle elongation velocity [Vpole] KMT shortening velocity [Vch-Vpole] 
 µm/min µm/min µm  µm µm/min µm/min µm/min 
Control 
    M 1.2a 0.4a 1.0a 1.1 0.9a 1.7 0.9 0.8 
    SD 0.4 0.4 0.2 0.6 0.1 0.5 0.2 0.4 
    IQR 0.6 0.5 0.2 0.8 0.2 0.7 0.2 0.6 
    n 392 tracks; 23 cells 43 tracks; 8 cells 207 tracks; 34 cells 59 tracks; 9 cells 198 tracks; 21 cells 88 tracks; 30 cells 30 cells 88 tracks; 30 cells 
Control + colchicine 
    M   0.7      
    SD   0.1      
    IQR   0.2      
    n   56 tracks; 8 cells      
Control + MG132 
    M   1.1a 1.2     
    SD   0.2 0.6     
    IQR   0.4 0.7     
    n   125 tracks; 20 cells 57 tracks; 9 cells     
Control (Prophase) 
    M     0.4a    
    SD     0.1    
    IQR     0.2    
    n     n = 102; 3 cells    
KLP10A RNAi 
    M 0.2a  0.9a 1.6 0.8a 1.3 0.4 0.9a 
    SD 0.3  0.2 0.9 0.1 0.6 0.2 0.6 
    IQR 0.2  0.2 1.2 0.2 0.9 0.3 0.9 
    n 105 tracks; 6 cells  137 tracks; 16 cells 50 tracks; 8 cells 267 tracks; 25 cells 186 tracks; 32 cells 32 cells 186 tracks; 32 cells 
KLP10A RNAi + MG132 
    M   0.9      
    SD   0.2      
    IQR   0.2      
    n   50 tracks; 7 cells      
KLP10A RNAi + (Prophase) 
    M     0.4a    
    SD     0.1    
    IQR     0.1    
    n     n = 79; 3 cells    
CLASP/KLP10A RNAi 
    M 0.2a 0.2a 1.0 1.7a 0.8a 1.1 0.4 0.7a 
    SD 0.4 0.4 0.2 1.7 0.2 0.4 0.2 0.4 
    IQR 0.6 0.4 0.2 1.4 0.2 0.6 0.2 0.6 
    n 208 tracks; 13 cells 51 tracks; 10 cells 159 tracks; 23 cells 51 tracks; 9 cells 241 tracks; 25 cells 219 tracks; 33 cells 33 cells 219 tracks; 33 cells 
CLASP/KLP10A RNAi + MG132 
    M   1.1a 1.4a     
    SD   0.2 0.7     
    IQR   0.2 1.0     
    n   129 tracks; 20 cells 44 tracks; 8 cells     
CLASP/KLP10A RNAi (Prophase) 
    M     0.4a    
    SD     0.1    
    IQR     0.1    
    n     22 tracks; 2 cells    
Mad2 RNAi 
    M     0.8a    
    SD     0.2    
    IQR     0.2    
    n     122 tracks; 12 cels    
Mad2 RNAi (Prophase) 
    M     0.4a    
    SD     0.1    
    IQR     0.1    
    n     54 tracks; 3 cells    
KLP10A/SAS4 RNAi 
    M      1.6 0.7 0.9 
    SD      0.6 0.2 0.5 
    IQR      0.6 0.2 0.7 
    n      84 tracks; 14 cells 14 cells 84 tracks; 14 cells 
Taxol 
    M 0.1a  0.7 3.3a     
    SD 0.2  0.1 2.0     
    IQR 0.2  0.1 3.4     
    n 73 tracks; 5 cells  44 tracks; 7 cells 42 tracks; 7 cells     
Experiment Metaphase flux velocity Anaphase kMT minus-end depolymerization velocity Inter-KT distance measured from fixed cells Pixel intensity BubR1/CID Inter-KT distance measured from live imaging Chromosome poleward velocity [Vch] Half-spindle elongation velocity [Vpole] KMT shortening velocity [Vch-Vpole] 
 µm/min µm/min µm  µm µm/min µm/min µm/min 
Control 
    M 1.2a 0.4a 1.0a 1.1 0.9a 1.7 0.9 0.8 
    SD 0.4 0.4 0.2 0.6 0.1 0.5 0.2 0.4 
    IQR 0.6 0.5 0.2 0.8 0.2 0.7 0.2 0.6 
    n 392 tracks; 23 cells 43 tracks; 8 cells 207 tracks; 34 cells 59 tracks; 9 cells 198 tracks; 21 cells 88 tracks; 30 cells 30 cells 88 tracks; 30 cells 
Control + colchicine 
    M   0.7      
    SD   0.1      
    IQR   0.2      
    n   56 tracks; 8 cells      
Control + MG132 
    M   1.1a 1.2     
    SD   0.2 0.6     
    IQR   0.4 0.7     
    n   125 tracks; 20 cells 57 tracks; 9 cells     
Control (Prophase) 
    M     0.4a    
    SD     0.1    
    IQR     0.2    
    n     n = 102; 3 cells    
KLP10A RNAi 
    M 0.2a  0.9a 1.6 0.8a 1.3 0.4 0.9a 
    SD 0.3  0.2 0.9 0.1 0.6 0.2 0.6 
    IQR 0.2  0.2 1.2 0.2 0.9 0.3 0.9 
    n 105 tracks; 6 cells  137 tracks; 16 cells 50 tracks; 8 cells 267 tracks; 25 cells 186 tracks; 32 cells 32 cells 186 tracks; 32 cells 
KLP10A RNAi + MG132 
    M   0.9      
    SD   0.2      
    IQR   0.2      
    n   50 tracks; 7 cells      
KLP10A RNAi + (Prophase) 
    M     0.4a    
    SD     0.1    
    IQR     0.1    
    n     n = 79; 3 cells    
CLASP/KLP10A RNAi 
    M 0.2a 0.2a 1.0 1.7a 0.8a 1.1 0.4 0.7a 
    SD 0.4 0.4 0.2 1.7 0.2 0.4 0.2 0.4 
    IQR 0.6 0.4 0.2 1.4 0.2 0.6 0.2 0.6 
    n 208 tracks; 13 cells 51 tracks; 10 cells 159 tracks; 23 cells 51 tracks; 9 cells 241 tracks; 25 cells 219 tracks; 33 cells 33 cells 219 tracks; 33 cells 
CLASP/KLP10A RNAi + MG132 
    M   1.1a 1.4a     
    SD   0.2 0.7     
    IQR   0.2 1.0     
    n   129 tracks; 20 cells 44 tracks; 8 cells     
CLASP/KLP10A RNAi (Prophase) 
    M     0.4a    
    SD     0.1    
    IQR     0.1    
    n     22 tracks; 2 cells    
Mad2 RNAi 
    M     0.8a    
    SD     0.2    
    IQR     0.2    
    n     122 tracks; 12 cels    
Mad2 RNAi (Prophase) 
    M     0.4a    
    SD     0.1    
    IQR     0.1    
    n     54 tracks; 3 cells    
KLP10A/SAS4 RNAi 
    M      1.6 0.7 0.9 
    SD      0.6 0.2 0.5 
    IQR      0.6 0.2 0.7 
    n      84 tracks; 14 cells 14 cells 84 tracks; 14 cells 
Taxol 
    M 0.1a  0.7 3.3a     
    SD 0.2  0.1 2.0     
    IQR 0.2  0.1 3.4     
    n 73 tracks; 5 cells  44 tracks; 7 cells 42 tracks; 7 cells     

Blank cells indicate that the value was not determined. KT, kinetochore; M, represents the mean, except for the distributions that do not follow a normal distribution; IQR, interquartile range; n, the number of measurements (in the prophase situation, n represents the number of interkinetochore distances measured before nuclear envelope breakdown).

a

Distrubutions that do not follow a normal distribution, where median is represented.

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