Effect of AID deficiency of IgH locus stability in activated B cells deficient for DNA-PKcs, Artemis, or ATM
| Genotype | No. mice (no. metaphases) | % Metaphases with IgH locus breaks (ave ± SD) |
|---|---|---|
| DNA-PKcs/AID | ||
| HL | 3 (150) | 2.0 ± 2.0 |
| AID−/−/HC/LC | 2 (100) | 0.0 ± 0.0 |
| DNA-PKcs−/−/HC/LC | 3 (150) | 15.3 ± 3.1 |
| DNA-PKcs−/−/ AID−/−/HC/LC | 2 (100) | 0.0 ± 0.0 |
| Artemis/AID | ||
| HL | 2 (100) | 1.0 ± 1.4 |
| AID−/−/HC/LC | 2 (100) | 0.0 ± 0.0 |
| Artemis−/−/HC/LC | 6 (300) | 9.7 ± 2.7 |
| Artemis−/−/ AID−/−/HC/LC | 3 (150) | 0.0 ± 0.0 |
| ATM/AID | ||
| wild-type | 2 (100) | 0.0 ± 0.0 |
| AID−/− | 1 (50) | 0.0 ± 0.0 |
| ATM−/− | 3 (150) | 18.0 ± 4.0 |
| ATM−/−/ AID−/− | 3 (150) | 8.0 ± 2.0 |
| Genotype | No. mice (no. metaphases) | % Metaphases with IgH locus breaks (ave ± SD) |
|---|---|---|
| DNA-PKcs/AID | ||
| HL | 3 (150) | 2.0 ± 2.0 |
| AID−/−/HC/LC | 2 (100) | 0.0 ± 0.0 |
| DNA-PKcs−/−/HC/LC | 3 (150) | 15.3 ± 3.1 |
| DNA-PKcs−/−/ AID−/−/HC/LC | 2 (100) | 0.0 ± 0.0 |
| Artemis/AID | ||
| HL | 2 (100) | 1.0 ± 1.4 |
| AID−/−/HC/LC | 2 (100) | 0.0 ± 0.0 |
| Artemis−/−/HC/LC | 6 (300) | 9.7 ± 2.7 |
| Artemis−/−/ AID−/−/HC/LC | 3 (150) | 0.0 ± 0.0 |
| ATM/AID | ||
| wild-type | 2 (100) | 0.0 ± 0.0 |
| AID−/− | 1 (50) | 0.0 ± 0.0 |
| ATM−/− | 3 (150) | 18.0 ± 4.0 |
| ATM−/−/ AID−/− | 3 (150) | 8.0 ± 2.0 |
B cells of the indicated genotypes were activated with α-CD40/IL-4 for 4 d, and metaphase spreads were hybridized with BAC probes flanking IgH (3′ and 5′ IgH). IgH locus-specific breaks (end-dissociated probes) were scored in 50 metaphases per culture. See Table S3 for data on individual cultures.
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