Summary of tertiary transplant results
| Secondary number | Positive proportion of tertiary recipients transplanted with | ||
| 100 LSK48−150+ | 40 LSK48−150+ | 20 LSK48−150+ | |
| #1 | 0 of 0 | 0 of 0 | 0 of 4 |
| #2 | 1 of 4 | 0 of 0 | 1 of 12 |
| #3 | 1 of 9 | 0 of 3 | 4 of 26 |
| #4 | 1 of 1 | 3 of 6 | 2 of 8 |
| #5 | 2 of 6 | 0 of 0 | 4 of 9 |
| #6 | 6 of 6 | 0 of 0 | 7 of 12 |
| #7 | 8 of 8 | 3 of 3 | 5 of 15 |
| Secondary number | Positive proportion of tertiary recipients transplanted with | ||
| 100 LSK48−150+ | 40 LSK48−150+ | 20 LSK48−150+ | |
| #1 | 0 of 0 | 0 of 0 | 0 of 4 |
| #2 | 1 of 4 | 0 of 0 | 1 of 12 |
| #3 | 1 of 9 | 0 of 3 | 4 of 26 |
| #4 | 1 of 1 | 3 of 6 | 2 of 8 |
| #5 | 2 of 6 | 0 of 0 | 4 of 9 |
| #6 | 6 of 6 | 0 of 0 | 7 of 12 |
| #7 | 8 of 8 | 3 of 3 | 5 of 15 |
6-12 mo after secondary transplantation of young LSK48−34−E+150+ cells, regenerated donor LSK48−150+ cells were purified from selected positive secondary recipients (#1–#7) and injected at limiting dilutions (20, 40, or 100 cells) into tertiary recipients, as indicated in Fig. 3 and described in Materials and methods. Shown in the table are the proportion of tertiary transplanted animals in which continuing donor-derived hematopoiesis could be detected (>0.5% donor contribution to circulating Gr-1+SSChi granulocytes at 16-24 wk after transplant). Repopulation details of all positive tertiary recipients and the corresponding primary and secondary recipients are shown in Fig. 6.