Table I.

Clinical manifestations

Induction + Prevention Alopecia2 mo Alopecia6 mo Diarrhea2 mo Diarrhea6 mo 
PBS + PBS No No 
CD4 + CD45RBhigh + PBS — Yes — 
CD4 + CD45RBhigh + iT reg cells B–C No No 
Induction + Prevention Alopecia2 mo Alopecia6 mo Diarrhea2 mo Diarrhea6 mo 
PBS + PBS No No 
CD4 + CD45RBhigh + PBS — Yes — 
CD4 + CD45RBhigh + iT reg cells B–C No No 

C.B-17 scid (BALB/c) mice were transferred with allogeneic C57BL/6 CD4+CD45RBhiCD25CD45.1+ cells (induction) along with PBS or anti-Balb iT reg cells (prevention). Control mice were injected twice with PBS (PBS + PBS). Skin manifestations and diarrhea were evaluated 2 and 6 mo later. Grading of skin involvement: A, no involvement; B, involvement of mouth or nose; C, involvement of back. Diarrhea was graded as yes or no. — denotes that this mouse group did not survive.

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