Data collection and structure refinement statistics
| Data collection | NKp30–B7-H6 | B7-H6 |
| Space group | P212121 | P21 |
| Unit cells (Å, o) | a = 50.9, b = 74.9, c = 125.5 | a = 44.3, b = 70.6, c = 53.2, γ = 100.4° |
| Resolution (Å)a | 50–2.3 (2.38–2.30) | 50–2.0 (2.07–2.00) |
| Observations | 299,606 | 159,065 |
| Unique reflections | 21,751 | 21,805 |
| Completeness (%)a | 98.4 (88.2) | 100 (99.6) |
| Mean I/σ(I)a | 53.4 (3.3) | 58.0 (2.6) |
| Rsym (%)ab | 6.5 (48.5) | 5.7 (47.6) |
| Refinement | ||
| Resolution range (Å) | 50–2.30 | 50–2.0 |
| Rwork (%)c | 23.9 | 24.2 |
| Rfree (%)c | 28.9 | 28.9 |
| Protein atoms | 2,543 | 1,665 |
| Carbohydrate atoms | 56 | 70 |
| Water molecules | 16 | 32 |
| r.m.s. deviations from ideality | ||
| Bond lengths (Å) | 0.019 | 0.019 |
| Bond angles (°) | 1.99 | 1.88 |
| Ramachandran statistics (%) | ||
| Favorable | 85.5 | 93.3 |
| Additional | 12.7 | 5.6 |
| Generous | 1.4 | 1.1 |
| Forbidden | 0.4 | 0.0 |
| Data collection | NKp30–B7-H6 | B7-H6 |
| Space group | P212121 | P21 |
| Unit cells (Å, o) | a = 50.9, b = 74.9, c = 125.5 | a = 44.3, b = 70.6, c = 53.2, γ = 100.4° |
| Resolution (Å)a | 50–2.3 (2.38–2.30) | 50–2.0 (2.07–2.00) |
| Observations | 299,606 | 159,065 |
| Unique reflections | 21,751 | 21,805 |
| Completeness (%)a | 98.4 (88.2) | 100 (99.6) |
| Mean I/σ(I)a | 53.4 (3.3) | 58.0 (2.6) |
| Rsym (%)ab | 6.5 (48.5) | 5.7 (47.6) |
| Refinement | ||
| Resolution range (Å) | 50–2.30 | 50–2.0 |
| Rwork (%)c | 23.9 | 24.2 |
| Rfree (%)c | 28.9 | 28.9 |
| Protein atoms | 2,543 | 1,665 |
| Carbohydrate atoms | 56 | 70 |
| Water molecules | 16 | 32 |
| r.m.s. deviations from ideality | ||
| Bond lengths (Å) | 0.019 | 0.019 |
| Bond angles (°) | 1.99 | 1.88 |
| Ramachandran statistics (%) | ||
| Favorable | 85.5 | 93.3 |
| Additional | 12.7 | 5.6 |
| Generous | 1.4 | 1.1 |
| Forbidden | 0.4 | 0.0 |
Values in parentheses are statistics for the highest resolution shells.
Rsym = ∑|Ij − <I>|/∑Ij, where Ij is the intensity of an individual reflection and <I> is the average intensity of that reflection.
Rwork = ∑‖Fo| − |Fc‖/∑|Fo|, where Fc is the calculated structure factor. Rfree is as for Rwork but calculated for a randomly selected 5.0% of reflections not included in the refinement.