Analysis of T cell populations in Cre/PLCγ1fl/− and the control mice
| Thymocytes | Splenocytes | ||||||||
| Total | DN | DP | CD4 SP | CD8 SP | Total | CD4+ | CD8+ | ||
| PLCγ1+/− (n = 6) | % | 1.5 ± 0.8 | 87 ± 2.8 | 8.5 ± 1.5 | 2.7 ± 1.2 | 22 ± 3.6 | 15 ± 2.9 | ||
| # (×106) | 216 ± 76 | 3.2 ± 2.0 | 189 ± 69 | 19 ± 8.3 | 6.4 ± 4.1 | 100 ± 46 | 22 ± 11 | 15 ± 6.6 | |
| PLCγ1fl/− (n = 6) | % | 1.1 ± 0.7 | 87 ± 4.1 | 9.4 ± 2.1 | 2.8 ± 1.5 | 18 ± 8.7 | 12 ± 5.1 | ||
| # (×106) | 238 ± 61 | 2.6 ± 1.5 | 223 ± 40 | 24 ± 6.4 | 6.9 ± 3.4 | 93 ± 42 | 17 ± 11 | 11 ± 7.6 | |
| Cre/PLCγ1+/− (n = 8) | % | 2 ± 0.8 | 87 ± 1.5 | 8.3 ± 1.5 | 2.9 ± 1.0 | 15 ± 5.3 | 12 ± 4.0 | ||
| # (×106) | 201 ± 88 | 3.6 ± 1.5 | 174 ± 72 | 17 ± 8.3 | 6.0 ± 4.0 | 118 ± 46 | 18 ± 8.6 | 13 ± 6.6 | |
| Cre/PLCγ1fl/− (n = 9) | % | 2.1 ± 1.2 | 95 ± 2.3a | 1.5 ± 0.43a | 1.2 ± 0.9b | 3.2 ± 1.5a | 5.6 ± 1.8c | ||
| # (×106) | 172 ± 81 | 3.7 ± 2.8 | 170 ± 72 | 2.7 ± 1.7c | 2.3 ± 2.0d | 77 ± 27 | 2.7 ± 2c | 4.1 ± 2.8b | |
| Thymocytes | Splenocytes | ||||||||
| Total | DN | DP | CD4 SP | CD8 SP | Total | CD4+ | CD8+ | ||
| PLCγ1+/− (n = 6) | % | 1.5 ± 0.8 | 87 ± 2.8 | 8.5 ± 1.5 | 2.7 ± 1.2 | 22 ± 3.6 | 15 ± 2.9 | ||
| # (×106) | 216 ± 76 | 3.2 ± 2.0 | 189 ± 69 | 19 ± 8.3 | 6.4 ± 4.1 | 100 ± 46 | 22 ± 11 | 15 ± 6.6 | |
| PLCγ1fl/− (n = 6) | % | 1.1 ± 0.7 | 87 ± 4.1 | 9.4 ± 2.1 | 2.8 ± 1.5 | 18 ± 8.7 | 12 ± 5.1 | ||
| # (×106) | 238 ± 61 | 2.6 ± 1.5 | 223 ± 40 | 24 ± 6.4 | 6.9 ± 3.4 | 93 ± 42 | 17 ± 11 | 11 ± 7.6 | |
| Cre/PLCγ1+/− (n = 8) | % | 2 ± 0.8 | 87 ± 1.5 | 8.3 ± 1.5 | 2.9 ± 1.0 | 15 ± 5.3 | 12 ± 4.0 | ||
| # (×106) | 201 ± 88 | 3.6 ± 1.5 | 174 ± 72 | 17 ± 8.3 | 6.0 ± 4.0 | 118 ± 46 | 18 ± 8.6 | 13 ± 6.6 | |
| Cre/PLCγ1fl/− (n = 9) | % | 2.1 ± 1.2 | 95 ± 2.3a | 1.5 ± 0.43a | 1.2 ± 0.9b | 3.2 ± 1.5a | 5.6 ± 1.8c | ||
| # (×106) | 172 ± 81 | 3.7 ± 2.8 | 170 ± 72 | 2.7 ± 1.7c | 2.3 ± 2.0d | 77 ± 27 | 2.7 ± 2c | 4.1 ± 2.8b | |
The age of the mice analyzed was between 3 and 10 wk. Data presented are average percentage (%) or absolute number (#) of each T cell subset. The P value was calculated by comparing the percentages or absolute numbers of different T cell subsets from Cre/PLCγ1fl/− mice to those from Cre/PLCγ1+/− mice. There was no significant difference in the percentages and absolute numbers of different T cell subsets from PLCγ1+/−, PLCγ1fl/−, and Cre/PLCγ1+/− mice.
P < 0.0001.
P < 0.01.
P < 0.001.
P < 0.05.