Effect of mutations of IS4 on inhibition of HIV-1 infection with NL-LucR.T2A-BaL.ecto in the PBMC assay
| Heavy chain | Light chain | Binding to cardiolipin and PS (Giles et al., 2006) | IC50 against B.BaL |
| µg/ml | |||
| IS4 IgG1 VH | IS4 VL | yes | 10.4 |
| IS4 IgG1 VH | B3 VL | yes | 7.4 |
| mutated IS4 IgG1 VHa | IS4 VL | no | >50 |
| Heavy chain | Light chain | Binding to cardiolipin and PS (Giles et al., 2006) | IC50 against B.BaL |
| µg/ml | |||
| IS4 IgG1 VH | IS4 VL | yes | 10.4 |
| IS4 IgG1 VH | B3 VL | yes | 7.4 |
| mutated IS4 IgG1 VHa | IS4 VL | no | >50 |
Mutations in IS4 heavy chain are arginine-to-serine at positions 96 and 97.