Table II.

Antigen specificities of autoreactive T cell lines

 TT PPD Flu MelanA 
Donor 1 n.d. n.d. 
Donor 2 − ++ ++ ++ 
Donor 3 ++ n.d. 
Donor 4 − 
Donor 5 n.d. n.d. n.d. 
 TT PPD Flu MelanA 
Donor 1 n.d. n.d. 
Donor 2 − ++ ++ ++ 
Donor 3 ++ n.d. 
Donor 4 − 
Donor 5 n.d. n.d. n.d. 

CCR6+ T cells that had completely lost CFSE labeling after stimulation with autologous DCs and anti–IL-10 antibodies for 7 d were sorted, expanded with recombinant IL-2, and tested for cytokine production (TNF, IFN-γ, or IL-2) after restimulation with autologous monocytes in the absence or presence of TT, purified protein derivate (PPD), influenza hemagglutinin (Flu), or MelanA. −, cytokine responses <0.1% over control; +, cytokine responses between 0.1 and 1% over control; and ++, cytokine responses >1% over control. n.d., not done.

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