Table I. Detection of Qa-1 b –directed... | Rockefeller University Press

Table I.

Detection of Qa-1^b–directed IFN-γ responses in ex vivo cultures

Target cells^a	Independent T cell culture^b
	1	2	3	4	5	6	7	8	9	10	11	12
RMA-S	5,723^c	10,113	1,204	6,582	12,199	4,896	2,894	10,389	1,359	11,593	4,320	1,567
C4.4-25	97	84	92	82	164	61	23	18	106	61	47	14
EC7.1	364	712	310	216	1,311	799	273	81	485	564	418	38
EC7.1.K^b	5,886	11,270	538	7,184	16,031	2,526	441	8,309	405	11,111	1,552	837
EC7.1.D^b	888	7,507	645	690	10,113	2,125	1,129	604	648	8,314	1,561	446
EC7.1.Qa-1^b	2,053	5,579	3,330	6,677	16,265	3,108	2,999	272	1,410	8,827	2,697	7,039
EC7.1.cont	380	700	386	250	1,370	1,065	418	63	591	620	401	56
Medium	287	120	287	152	276	156	177	38	218	110	74	13

Target cells^a	Independent T cell culture^b
	1	2	3	4	5	6	7	8	9	10	11	12
RMA-S	5,723^c	10,113	1,204	6,582	12,199	4,896	2,894	10,389	1,359	11,593	4,320	1,567
C4.4-25	97	84	92	82	164	61	23	18	106	61	47	14
EC7.1	364	712	310	216	1,311	799	273	81	485	564	418	38
EC7.1.K^b	5,886	11,270	538	7,184	16,031	2,526	441	8,309	405	11,111	1,552	837
EC7.1.D^b	888	7,507	645	690	10,113	2,125	1,129	604	648	8,314	1,561	446
EC7.1.Qa-1^b	2,053	5,579	3,330	6,677	16,265	3,108	2,999	272	1,410	8,827	2,697	7,039
EC7.1.cont	380	700	386	250	1,370	1,065	418	63	591	620	401	56
Medium	287	120	287	152	276	156	177	38	218	110	74	13

RMA-S is TAP2 deficient; C4.4-25 is β2m deficient; EC7.1 is a MHC class I–deficient variant of RMA-S. Single class I genes were reconstituted in EC7.1.

12 mice were immunized with RMA-S.B7 and spleens were stimulated twice in vitro with RMA-S.B7 before testing.

Concentration of IFN-γ released by ex vivo cultures (pg/ml).

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