SNPs in the region flanking CTGF are associated with severe HF in two Chinese samples
| Chinese fishermen | Chinese farmers | |||||||||||||
| SNP | Position | Bins | Genotype | Controls | Cases | OR | 95% CI | P | Controls | Cases | OR | 95% CI | P | |
| % | % | % | % | |||||||||||
| Univariate analysis | rs1257705 | 132304944 | II | AA | 69.1 | 83.7 | 2.26 | 1.13–4.5 | 0.02 | 65.1 | 72 | 1.67 | 0.98–2.84 | 0.06 |
| rs12526196 | 132305169 | II | TT | 67.9 | 82.5 | 2.21 | 1.13–4.4 | 0.02 | 61.6 | 71.8 | 1.85 | 1.1–3.1 | 0.02 | |
| rs9399005 | 132310657 | CC | 17.2 | 26.3 | 2.2 | 1.1–4.29 | 0.02 | NS | ||||||
| rs6918698 | 132314950 | III | CC | 26.6 | 35.1 | 2 | 1.1–3.7 | 0.02 | NS | |||||
| rs3037970 | 132316891 | III | −/− | 27.3 | 38.8 | 2.59 | 1.4–4.8 | 0.003 | NS | |||||
| rs1931002 | 132320175 | IV | CC | 52.5 | 66.7 | 2.32 | 1.3–4.13 | 0.004 | NS | |||||
| rs2151532 | 132316423 | IV | AA | 55.4 | 65 | 1.98 | 1.12–3.5 | 0.02 | NS | |||||
| rs9402373 | 132319124 | VI | CC | 50.3 | 60.6 | 2.04 | 1.15–3.54 | 0.015 | 25 | 43.1 | 2.23 | 1.32–3.76 | 0.003 | |
| Multivariate analysis | rs12526196 | II | TT | 3 | 1.4–6.6 | 0.007 | 1.88 | 1.13–3.19 | 0.018 | |||||
| rs9402373 | VI | CC | 2.8 | 1.5–5.4 | 0.002 | 2.26 | 1.33–3.83 | 0.03 | ||||||
| rs1931002 | IV | CC | 2.81 | 1.47–5.37 | 0.002 | |||||||||
| Chinese fishermen | Chinese farmers | |||||||||||||
| SNP | Position | Bins | Genotype | Controls | Cases | OR | 95% CI | P | Controls | Cases | OR | 95% CI | P | |
| % | % | % | % | |||||||||||
| Univariate analysis | rs1257705 | 132304944 | II | AA | 69.1 | 83.7 | 2.26 | 1.13–4.5 | 0.02 | 65.1 | 72 | 1.67 | 0.98–2.84 | 0.06 |
| rs12526196 | 132305169 | II | TT | 67.9 | 82.5 | 2.21 | 1.13–4.4 | 0.02 | 61.6 | 71.8 | 1.85 | 1.1–3.1 | 0.02 | |
| rs9399005 | 132310657 | CC | 17.2 | 26.3 | 2.2 | 1.1–4.29 | 0.02 | NS | ||||||
| rs6918698 | 132314950 | III | CC | 26.6 | 35.1 | 2 | 1.1–3.7 | 0.02 | NS | |||||
| rs3037970 | 132316891 | III | −/− | 27.3 | 38.8 | 2.59 | 1.4–4.8 | 0.003 | NS | |||||
| rs1931002 | 132320175 | IV | CC | 52.5 | 66.7 | 2.32 | 1.3–4.13 | 0.004 | NS | |||||
| rs2151532 | 132316423 | IV | AA | 55.4 | 65 | 1.98 | 1.12–3.5 | 0.02 | NS | |||||
| rs9402373 | 132319124 | VI | CC | 50.3 | 60.6 | 2.04 | 1.15–3.54 | 0.015 | 25 | 43.1 | 2.23 | 1.32–3.76 | 0.003 | |
| Multivariate analysis | rs12526196 | II | TT | 3 | 1.4–6.6 | 0.007 | 1.88 | 1.13–3.19 | 0.018 | |||||
| rs9402373 | VI | CC | 2.8 | 1.5–5.4 | 0.002 | 2.26 | 1.33–3.83 | 0.03 | ||||||
| rs1931002 | IV | CC | 2.81 | 1.47–5.37 | 0.002 | |||||||||
Data are provided for two independent Chinese samples (fishermen and farmers living in a region endemic for S. japonicum). Cases and controls were defined as indicated in Materials and methods and in Table S1. The association between genotypes and HF phenotypes were first tested separately (univariate analysis) and then simultaneously (multivariate analysis that included SNPS rs12526196, rs1931002, rs3037970, and rs9402373, which showed the strongest associations when tested against SNPs from the same bin). Bins represented correlation (r2 > 0.8) groups, and genotype was the aggravating genotype. In the Chinese fisherman sample (n = 300, 99 cases and 201 controls), the covariates were the number of years of fishing, being born on a boat, sex, and the number of Praziquantel treatments. In the Chinese farmer sample (n = 294, 113 controls and 181 cases), the covariates were age, whether HF was endemic or not endemic, and whether they had been infected with HBV (P = 0.05; OR = 2.38). P, p-value.