Table I. Bcl6 mutations in human PB B... | Rockefeller University Press

Table I.

Bcl6 mutations in human PB B cells

Donor	Cell type	PCR efficiency^a	1 allele/2 alleles amplified^b	Mutated cells	Number of mutations^c	Mutation frequency^d
				(%)		%
A	naive	26/50	22/4	0	0	0
	IgM⁺IgD⁺CD27⁺	31/50	22/9	7 (23)	6 × 1, 1 × 2	0.039
	IgM-only	26/50	21/5	5 (19)	5 × 1	0.024
	class switched	18/50	16/2	6 (33)	4 × 1, 1 × 2, 1 × 5	0.082
B	naive	18/50	11/7	0	0	0
	IgM⁺IgD⁺CD27⁺	25/50	21/4	5 (20)	4 × 1, 1 × 3	0.036
	IgM-only	20/50	15/5	6 (30)	1 × 1, 2 × 2, 1 × 4, 1 × 5, 1 × 6	0.119
	class switched	24/50	19/5	8 (33)	4 × 1, 2 × 2, 1 × 3, 1 × 5	0.082
C	naive	16/40	12/4	0	0	0
	IgM⁺IgD⁺CD27⁺	19/40	15/4	4 (21)	2 × 1, 1 × 5, 1 × 6	0.084
	IgM-only	18/40	13/5	2 (11)	2 × 1	0.013
	class switched	15/40	14/1	5 (33)	2 × 1, 2 × 2, 1 × 4	0.093
mean	naive	60/140	45/15	0	0	0
	IgM⁺IgD⁺CD27⁺	75/140	58/17	16 (21)	12 × 1, 1 × 2, 1 × 3, 1 × 5, 1 × 6	0.045
	IgM-only	64/140	49/15	13 (20)	8 × 1, 2 × 2, 1 × 4, 1 × 5, 1 × 6	0.050
	class switched	57/140	49/8	19 (33)	10 × 1, 5 × 2, 1 × 3, 1 × 4, 2 × 5	0.085

Donor	Cell type	PCR efficiency^a	1 allele/2 alleles amplified^b	Mutated cells	Number of mutations^c	Mutation frequency^d
				(%)		%
A	naive	26/50	22/4	0	0	0
	IgM⁺IgD⁺CD27⁺	31/50	22/9	7 (23)	6 × 1, 1 × 2	0.039
	IgM-only	26/50	21/5	5 (19)	5 × 1	0.024
	class switched	18/50	16/2	6 (33)	4 × 1, 1 × 2, 1 × 5	0.082
B	naive	18/50	11/7	0	0	0
	IgM⁺IgD⁺CD27⁺	25/50	21/4	5 (20)	4 × 1, 1 × 3	0.036
	IgM-only	20/50	15/5	6 (30)	1 × 1, 2 × 2, 1 × 4, 1 × 5, 1 × 6	0.119
	class switched	24/50	19/5	8 (33)	4 × 1, 2 × 2, 1 × 3, 1 × 5	0.082
C	naive	16/40	12/4	0	0	0
	IgM⁺IgD⁺CD27⁺	19/40	15/4	4 (21)	2 × 1, 1 × 5, 1 × 6	0.084
	IgM-only	18/40	13/5	2 (11)	2 × 1	0.013
	class switched	15/40	14/1	5 (33)	2 × 1, 2 × 2, 1 × 4	0.093
mean	naive	60/140	45/15	0	0	0
	IgM⁺IgD⁺CD27⁺	75/140	58/17	16 (21)	12 × 1, 1 × 2, 1 × 3, 1 × 5, 1 × 6	0.045
	IgM-only	64/140	49/15	13 (20)	8 × 1, 2 × 2, 1 × 4, 1 × 5, 1 × 6	0.050
	class switched	57/140	49/8	19 (33)	10 × 1, 5 × 2, 1 × 3, 1 × 4, 2 × 5	0.085

Numbers of positive PCRs versus single cells analyzed.

Number of PCR products containing one/two alleles.

In A × B, A denotes the number of sequences with B mutations.

The percentage of mutations in a total of 670 bp of the Bcl6-MMC considered from each sequence.