Impaired Ig secretion by STAT3MUT naive B cells in response to IL-10 and IL-21
| Ig secretion | ||||||||||
| IgM | IgG | IgA | ||||||||
| CD40L | +IL-21 | +IL-10 | +CpG | CD40L | +IL-21 | +IL-10 | CD40L | +IL-21 | +IL-10 | |
| ng/ml | ng/ml | ng/ml | ng/ml | ng/ml | ng/ml | ng/ml | ng/ml | ng/ml | ng/ml | |
| Control (n = 12) | 30 ± 10 | 30,646 ± 5,344 | 640 ± 213 | 3,223 ± 1,023 | <0.1 | 538 ± 193 | 3.8 ± 2.5 | <0.1 | 3,750 ± 1,440 | 117 ± 81 |
| STAT3MUT (n = 7) | 33 ± 15 | 299 ± 168 | 47 ± 20 | 2,382 ± 546 | <0.1 | 4.9 ± 1.6 | <0.1 | <0.1 | 1.8 ± 1.7 | 7.5 ± 7.5 |
| HIES STAT3WT (n = 4) | 25 ± 13 | 4,125 ± 955 | ND | ND | <0.1 | 113 ± 67 | ND | <0.1 | 785 ± 484 | ND |
| STAT1MUT (n = 4) | 45 ± 43 | 20,706 ± 7,569 | 715 ± 555 | 5,314 ± 3,399 | <0.1 | 214 ± 114 | ND | <0.1 | 432 ± 275 | ND |
| Ig secretion | ||||||||||
| IgM | IgG | IgA | ||||||||
| CD40L | +IL-21 | +IL-10 | +CpG | CD40L | +IL-21 | +IL-10 | CD40L | +IL-21 | +IL-10 | |
| ng/ml | ng/ml | ng/ml | ng/ml | ng/ml | ng/ml | ng/ml | ng/ml | ng/ml | ng/ml | |
| Control (n = 12) | 30 ± 10 | 30,646 ± 5,344 | 640 ± 213 | 3,223 ± 1,023 | <0.1 | 538 ± 193 | 3.8 ± 2.5 | <0.1 | 3,750 ± 1,440 | 117 ± 81 |
| STAT3MUT (n = 7) | 33 ± 15 | 299 ± 168 | 47 ± 20 | 2,382 ± 546 | <0.1 | 4.9 ± 1.6 | <0.1 | <0.1 | 1.8 ± 1.7 | 7.5 ± 7.5 |
| HIES STAT3WT (n = 4) | 25 ± 13 | 4,125 ± 955 | ND | ND | <0.1 | 113 ± 67 | ND | <0.1 | 785 ± 484 | ND |
| STAT1MUT (n = 4) | 45 ± 43 | 20,706 ± 7,569 | 715 ± 555 | 5,314 ± 3,399 | <0.1 | 214 ± 114 | ND | <0.1 | 432 ± 275 | ND |
Naive B cells from the indicated groups were cultured (10 x 103 cells/well/200 μl) with CD40L alone or together with IL-2, IL-10, or CpG. Ig secretion was measured after 10–12 d. The values are means ± SEM for the indicated number of donors with the exception of cultures of naive B cells from healthy donors or STAT3MUT patients stimulated with CD40L/IL-10 or CD40L/CpG (n = 5) and STAT1MUT patients stimulated with CD40L/IL-10 (n = 2). ND, not done.