Platelet activation in vivo in collagen plus epinephrine-challenged WT and MMP-2−/− mice
| Parameter | Collagen plus epinephrine | WT mice | MMP-2−/− mice | Reduction in MMP-2−/− versus WT mice | P-value |
| µl | % | % | % | ||
| P-selectin | 80 | 27.7 ± 2.5 (n = 5) | 28.6 ± 3.5 (n = 5) | 0 | NS |
| 60 | 21.3 ± 3.7 (n = 5) | 13.1 ± 1.6 (n = 5) | 38.5 | NS | |
| 40 | 10.5 ± 1.6 (n = 8) | 4.4 ± 0.7 (n = 8) | 58 | 0.01315 | |
| Platelet/leukocyte aggregates | 60 | 43.84 ± 0.79 (n = 6) | 36.2 ± 6.05 (n = 4) | 17 | NS |
| 40 | 11.2 ± 0.9 (n = 10) | 8 ± 0.9 (n = 10) | 27.3 | 0.015 | |
| Fibrinogen binding | 80 | 39.6 ± 3.67 (n = 5) | 34.7 ± 4.01 (n = 5) | 12.4 | NS |
| 60 | 32 ± 0.91 (n = 5) | 24 ± 1.06 (n = 5) | 25 | 0.00128 | |
| 40 | 12.8 ± 1.62 (n = 5) | 8.5 ± 0.44 (n = 5) | 33.6 | 0.018 |
| Parameter | Collagen plus epinephrine | WT mice | MMP-2−/− mice | Reduction in MMP-2−/− versus WT mice | P-value |
| µl | % | % | % | ||
| P-selectin | 80 | 27.7 ± 2.5 (n = 5) | 28.6 ± 3.5 (n = 5) | 0 | NS |
| 60 | 21.3 ± 3.7 (n = 5) | 13.1 ± 1.6 (n = 5) | 38.5 | NS | |
| 40 | 10.5 ± 1.6 (n = 8) | 4.4 ± 0.7 (n = 8) | 58 | 0.01315 | |
| Platelet/leukocyte aggregates | 60 | 43.84 ± 0.79 (n = 6) | 36.2 ± 6.05 (n = 4) | 17 | NS |
| 40 | 11.2 ± 0.9 (n = 10) | 8 ± 0.9 (n = 10) | 27.3 | 0.015 | |
| Fibrinogen binding | 80 | 39.6 ± 3.67 (n = 5) | 34.7 ± 4.01 (n = 5) | 12.4 | NS |
| 60 | 32 ± 0.91 (n = 5) | 24 ± 1.06 (n = 5) | 25 | 0.00128 | |
| 40 | 12.8 ± 1.62 (n = 5) | 8.5 ± 0.44 (n = 5) | 33.6 | 0.018 |
P-selectin (expressed as percentage of positive platelets): basal value, 4.9 ± 0.8% of positive cells; Platelet/leukocyte aggregates (expressed as percentage of leukocytes bound to platelets): basal value, 6.3 ± 0.6% of leukocytes bound to platelets; Fibrinogen binding (expressed as percentage of positive platelets): basal value, 3.3 ± 0.14% of positive cells.