Medial remodeling of common carotid arteries after outflow reduction
. | WT . | . | MyD88−/− . | . | ||
|---|---|---|---|---|---|---|
| . | Right . | Left . | Right . | Left . | ||
| Medial lamellar units (number) | 3.0 ± 0 | 2.4 ± 0.2a | 3.0 ± 0 | 3.0 ± 0 | ||
| Inner lamella nuclei (per x-sec) | 39.2 ± 1.5 | 38.8 ± 0.6 | 41.0 ± 1.1 | 43.2 ± 1.0 | ||
| Middle lamella nuclei (per x-sec) | 36.8 ± 1.6 | 36.7 ± 1.3 | 36.2 ± 1.2 | 39.7 ± 1.4a | ||
| Outer lamella nuclei (per x-sec) | 23.8 ± 2.0 | 17.8 ± 2.8a | 27.9 ± 1.1 | 25.2 ± 2.6 | ||
| Medial nuclei density (per μm2 × 103) | 5.0 ± 0.2 | 4.9 ± 0.2 | 4.1 ± 0.1 | 4.7 ± 0.1 | ||
| Inner lamella SMC size (μm) | 6.0 ± 0.1 | 5.9 ± 0.1 | 6.5 ± 0.3 | 6.7 ± 0.2 | ||
| Middle lamella SMC size (μm) | 5.6 ± 0.2 | 5.7 ± 0.2 | 6.4 ± 0.2 | 6.5 ± 0.3 | ||
| Outer lamella SMC size (μm) | 3.7 ± 0.2 | 3.4 ± 0.3 | 5.1 ± 0.2 | 4.7 ± 0.3 | ||
| Medial α-SMA+ area (μm2 × 103) | 17.1 ± 0.7 | 14.7 ± 0.3a | 17.5 ± 0.9 | 16.1 ± 0.5 | ||
| Medial elastin+ area (μm2 × 103) | 13.1 ± 0.5 | 12.4 ± 0.5 | 12.9 ± 0.7 | 13.0 ± 0.8 | ||
| Medial sirius red+ area (μm2 × 103) | 9.6 ± 0.6 | 9.6 ± 0.7 | 12.0 ± 1.2 | 13.0 ± 0.7 | ||
. | WT . | . | MyD88−/− . | . | ||
|---|---|---|---|---|---|---|
| . | Right . | Left . | Right . | Left . | ||
| Medial lamellar units (number) | 3.0 ± 0 | 2.4 ± 0.2a | 3.0 ± 0 | 3.0 ± 0 | ||
| Inner lamella nuclei (per x-sec) | 39.2 ± 1.5 | 38.8 ± 0.6 | 41.0 ± 1.1 | 43.2 ± 1.0 | ||
| Middle lamella nuclei (per x-sec) | 36.8 ± 1.6 | 36.7 ± 1.3 | 36.2 ± 1.2 | 39.7 ± 1.4a | ||
| Outer lamella nuclei (per x-sec) | 23.8 ± 2.0 | 17.8 ± 2.8a | 27.9 ± 1.1 | 25.2 ± 2.6 | ||
| Medial nuclei density (per μm2 × 103) | 5.0 ± 0.2 | 4.9 ± 0.2 | 4.1 ± 0.1 | 4.7 ± 0.1 | ||
| Inner lamella SMC size (μm) | 6.0 ± 0.1 | 5.9 ± 0.1 | 6.5 ± 0.3 | 6.7 ± 0.2 | ||
| Middle lamella SMC size (μm) | 5.6 ± 0.2 | 5.7 ± 0.2 | 6.4 ± 0.2 | 6.5 ± 0.3 | ||
| Outer lamella SMC size (μm) | 3.7 ± 0.2 | 3.4 ± 0.3 | 5.1 ± 0.2 | 4.7 ± 0.3 | ||
| Medial α-SMA+ area (μm2 × 103) | 17.1 ± 0.7 | 14.7 ± 0.3a | 17.5 ± 0.9 | 16.1 ± 0.5 | ||
| Medial elastin+ area (μm2 × 103) | 13.1 ± 0.5 | 12.4 ± 0.5 | 12.9 ± 0.7 | 13.0 ± 0.8 | ||
| Medial sirius red+ area (μm2 × 103) | 9.6 ± 0.6 | 9.6 ± 0.7 | 12.0 ± 1.2 | 13.0 ± 0.7 | ||
Common carotid arteries were formalin fixed and paraffin embedded at 14 d after left external carotid artery ligation in WT and MyD88−/− mice (n = 9). The number of medial lamellar units continuous around >50% of the vessel circumference and the number of nuclei in each lamella were counted from H&E-stained cross sections (x-sec). The transverse diameter of SMCs was measured across the nucleus in sections immunostained for α-SMA. The medial area staining positive for α-SMA, elastin, or Sirius red was calculated using image analysis software. Data are means ± SE.
P < 0.05, Left versus Right.