Human oligonucleotide primers used for quantitative real-time RT-PCR
| Gene . | Primer Sequence . | Strand . | Product size . | Temperature . | Reference . |
|---|---|---|---|---|---|
| bp | °C | ||||
| SIK | 5′-CAACCTGGGCGACTACGATGAGCA-3′ | + | 167 | 60 | NM_173354 |
| 5′-GGGCGCACTGGGCATTCCGATACT-3′ | − | ||||
| CDKN1B (p15) | 5′-TGGACCTGGTGGCTACGAAT-3′(+) | + | 150 | 60 | Niimi et al. (2007) |
| 5′-AGGGCCTAAGTTGTGGGTTCA-3′(−) | − | ||||
| CDKN1A (p21) | 5′-CTGCCCAAGCTCTACCTTCC-3′(+) | + | 123 | 60 | Niimi et al. (2007) |
| 5′-CAGGTCCACATGGTCTTCCT-3′(−) | − | ||||
| PAI-1 | 5′-GAGACAGGCAGCTCGGATTC-3′ | + | 101 | 60 | ENST00000223095 |
| 5′-GGCCTCCCAAAGTGCATTAC-3′ | − | ||||
| MADH7 (Smad7) | 5′-ACCCGATGGATTTTCTCAAACC-3′ | + | 85 | 60 | ENST00000262158 |
| 5′-GCCAGATAATTCGTTCCCCCT-3′ | − | ||||
| GAPDH | 5′-GGAGTCAACGGATTTGGTCGTA-3′(+) | + | 78 | 60 | Niimi et al. (2007) |
| 5′-GGCAACAATATCCACTTTACCAGAGT-3′(−) | − |
| Gene . | Primer Sequence . | Strand . | Product size . | Temperature . | Reference . |
|---|---|---|---|---|---|
| bp | °C | ||||
| SIK | 5′-CAACCTGGGCGACTACGATGAGCA-3′ | + | 167 | 60 | NM_173354 |
| 5′-GGGCGCACTGGGCATTCCGATACT-3′ | − | ||||
| CDKN1B (p15) | 5′-TGGACCTGGTGGCTACGAAT-3′(+) | + | 150 | 60 | Niimi et al. (2007) |
| 5′-AGGGCCTAAGTTGTGGGTTCA-3′(−) | − | ||||
| CDKN1A (p21) | 5′-CTGCCCAAGCTCTACCTTCC-3′(+) | + | 123 | 60 | Niimi et al. (2007) |
| 5′-CAGGTCCACATGGTCTTCCT-3′(−) | − | ||||
| PAI-1 | 5′-GAGACAGGCAGCTCGGATTC-3′ | + | 101 | 60 | ENST00000223095 |
| 5′-GGCCTCCCAAAGTGCATTAC-3′ | − | ||||
| MADH7 (Smad7) | 5′-ACCCGATGGATTTTCTCAAACC-3′ | + | 85 | 60 | ENST00000262158 |
| 5′-GCCAGATAATTCGTTCCCCCT-3′ | − | ||||
| GAPDH | 5′-GGAGTCAACGGATTTGGTCGTA-3′(+) | + | 78 | 60 | Niimi et al. (2007) |
| 5′-GGCAACAATATCCACTTTACCAGAGT-3′(−) | − |
A previously published reference, the NCBI database accession number, or the Ensembl database transcript (ENST) number is indicated.