Mutations spectra observed in Canr mutant colonies from 7-d-old wild-type, sch9Δ, and rev1Δ cultures
| Strains | Clone | Type | Base change | Mutations | Position from ATG | Sequence |
| WTa | 1 | Base substitution | C → T | Proline-leucine | 656 | 5′-TGTTCCCTGTC-3′ |
| WTa | 2 | Base substitution | C → T | Proline-serine | 937 | 5′-GCTGCAAACCCC-3′ |
| WTa | 3 | Base substitution | T → G | Asparagine-lysine | 1,173 | 5′-AAATTCAAATA-3′ |
| WTa | 3 | Insertion | G | Frameshift | 1,710 | 5′-GAGGCGAATT-3′ |
| WTa | 4 | Base substitution | G → C | Alanine-proline | 709 | 5′-TTTTAGCCATTA-3′ |
| WTa | 4 | Deletion | TG | Frameshift | 1,098–1,099 | 5′-TTGCTATTGAGA-3′ |
| WTa | 5 | Base substitution | G → T | Glycine-valine | 353 | 5′-ACGCCGGCCCA-3′ |
| WTa | 5 | Insertion | A | Frameshift | 1,341 | 5′-CAAAAGTTTTC-3′ |
| WTa | 6 | Deletion | AT | Frameshift | 1,129–1,130 | 5′-GTCACATATCTT-3′ |
| WTa | 6 | Insertion | T | Frameshift (T3-T4) | 1,086 | 5′-CCTTTTATTATT-3′ |
| WTa | 7 | Base substitution | G → T | Tryptophan-cysteine | 531 | 5′-GTTTTCTTGGCA-3′ |
| WTa | 7 | Deletion | A | Frame-shift (A3-A2) | 663 | 5′-AAATATTACGGT-3′ |
| WTa | 7 | Insertion | T | Frameshift (T3-T4) | 1,086 | 5′-CCTTTTATTATT-3′ |
| WTa | 8 | Base substitution | G → A | Glutamic A–lysine | 679 | 5′-AATTCGAGTTCT-3′ |
| WTa | 8 | Base substitution | G → T | Valine-phenylalanine | 907 | 5′-AACTATTTGGTA-3′ |
| WTa | 8 | Deletion | A | Frameshift | 1,217 | 5′-ATCAAAGAACAC-3′ |
| WTa | 9 | Duplication | NA | 248 bp | 184–431 | NA |
| WTa | 10 | No PCR | NA | NA | NA | NA |
| sch9Δ | 1 | Base substitution | C → A | Serine-STOP | 11 | 5′-AAATTCAAAAG-3′ |
| sch9Δ | 2 | Base substitution | C → A | Serine-STOP | 11 | 5′-AAATTCAAAAG-3′ |
| sch9Δ | 3 | Base substitution | C → A | Serine-STOP | 11 | 5′-AAATTCAAAAG-3′ |
| sch9Δ | 4 | Base substitution | C → A | Serine-STOP | 11 | 5′-AAATTCAAAAG-3′ |
| sch9Δ | 5 | Base substitution | C → A | Serine-STOP | 1,244 | 5′-CTGTCAAGGAC-3′ |
| sch9Δ | 6 | Base substitution | T → C | Phenylalanine-leucine | 682 | 5′-ATTCGAGTTCT-3′ |
| sch9Δ | 7 | Insertion | T | Frameshift (T6-T7) | 1,386 | 5′-CTTTTTTTGCAT-3′ |
| sch9Δ | 8 | Base substitution | T → G | Isoleucine-serine | 305 | 5′-GGTACTATTGGT-3′ |
| sch9Δ | 8 | Insertion | T | Frameshift | 1,013 | 5′-TATTATTTCATT-3′ |
| sch9Δ | 9 | Base substitution | C → A | Serine-STOP | 11 | 5′-AAATTCAAAAG-3′ |
| sch9Δ | 9 | Base substitution | C → A | No change | 1,347 | 5′-TTTTCGAATGG-3′ |
| sch9Δ | 10 | Insertion | T | Frameshift (T4-T5) | 1,022 | 5′-GGACTTTTTAGT-3′ |
| sch9Δ | 10 | Insertion | T | Frameshift (T6-T7) | 1,386 | 5′-CTTTTTTTGCAT-3′ |
| rev1Δ | 1 | Base substitution | C → T | Arginine-serine | 1,195 | 5′-GGTTCCCGTAT-3′ |
| rev1Δ | 2 | Insertion | A | Frameshift (A3-A4) | 702 | 5′-CAAAAGTTTA-3′ |
| rev1Δ | 3 | Deletion | G | Frameshift | 422 | 5′-GGGTGAAATGG-3′ |
| rev1Δ | 3 | Deletion | AAA | A3-A0 | 425-427 | 5′-GGGTGAAATGG-3′ |
| rev1Δ | 4 | Deletion | G | Frameshift | 422 | 5′-GGGTGAAATGG-3′ |
| rev1Δ | 4 | Deletion | AAA | A3-A0 | 425-427 | 5′-GGGTGAAATGG-3′ |
| rev1Δ | 5 | No PCR | NA | NA | NA | NA |
| rev1Δ | 6 | Base substitution | G → A | Tryptophan-STOP | 522 | 5′-GTATTGGTTTTC-3′ |
| rev1Δ | 7 | Base substitution | C → T | Serine-leucine | 1,214 | 5′-TATCAAAGAAC-3′ |
| rev1Δ | 8 | Base substitution | C → T | Histidine-tyrosine | 274 | 5′-CAAAGACATATT-3′ |
| Strains | Clone | Type | Base change | Mutations | Position from ATG | Sequence |
| WTa | 1 | Base substitution | C → T | Proline-leucine | 656 | 5′-TGTTCCCTGTC-3′ |
| WTa | 2 | Base substitution | C → T | Proline-serine | 937 | 5′-GCTGCAAACCCC-3′ |
| WTa | 3 | Base substitution | T → G | Asparagine-lysine | 1,173 | 5′-AAATTCAAATA-3′ |
| WTa | 3 | Insertion | G | Frameshift | 1,710 | 5′-GAGGCGAATT-3′ |
| WTa | 4 | Base substitution | G → C | Alanine-proline | 709 | 5′-TTTTAGCCATTA-3′ |
| WTa | 4 | Deletion | TG | Frameshift | 1,098–1,099 | 5′-TTGCTATTGAGA-3′ |
| WTa | 5 | Base substitution | G → T | Glycine-valine | 353 | 5′-ACGCCGGCCCA-3′ |
| WTa | 5 | Insertion | A | Frameshift | 1,341 | 5′-CAAAAGTTTTC-3′ |
| WTa | 6 | Deletion | AT | Frameshift | 1,129–1,130 | 5′-GTCACATATCTT-3′ |
| WTa | 6 | Insertion | T | Frameshift (T3-T4) | 1,086 | 5′-CCTTTTATTATT-3′ |
| WTa | 7 | Base substitution | G → T | Tryptophan-cysteine | 531 | 5′-GTTTTCTTGGCA-3′ |
| WTa | 7 | Deletion | A | Frame-shift (A3-A2) | 663 | 5′-AAATATTACGGT-3′ |
| WTa | 7 | Insertion | T | Frameshift (T3-T4) | 1,086 | 5′-CCTTTTATTATT-3′ |
| WTa | 8 | Base substitution | G → A | Glutamic A–lysine | 679 | 5′-AATTCGAGTTCT-3′ |
| WTa | 8 | Base substitution | G → T | Valine-phenylalanine | 907 | 5′-AACTATTTGGTA-3′ |
| WTa | 8 | Deletion | A | Frameshift | 1,217 | 5′-ATCAAAGAACAC-3′ |
| WTa | 9 | Duplication | NA | 248 bp | 184–431 | NA |
| WTa | 10 | No PCR | NA | NA | NA | NA |
| sch9Δ | 1 | Base substitution | C → A | Serine-STOP | 11 | 5′-AAATTCAAAAG-3′ |
| sch9Δ | 2 | Base substitution | C → A | Serine-STOP | 11 | 5′-AAATTCAAAAG-3′ |
| sch9Δ | 3 | Base substitution | C → A | Serine-STOP | 11 | 5′-AAATTCAAAAG-3′ |
| sch9Δ | 4 | Base substitution | C → A | Serine-STOP | 11 | 5′-AAATTCAAAAG-3′ |
| sch9Δ | 5 | Base substitution | C → A | Serine-STOP | 1,244 | 5′-CTGTCAAGGAC-3′ |
| sch9Δ | 6 | Base substitution | T → C | Phenylalanine-leucine | 682 | 5′-ATTCGAGTTCT-3′ |
| sch9Δ | 7 | Insertion | T | Frameshift (T6-T7) | 1,386 | 5′-CTTTTTTTGCAT-3′ |
| sch9Δ | 8 | Base substitution | T → G | Isoleucine-serine | 305 | 5′-GGTACTATTGGT-3′ |
| sch9Δ | 8 | Insertion | T | Frameshift | 1,013 | 5′-TATTATTTCATT-3′ |
| sch9Δ | 9 | Base substitution | C → A | Serine-STOP | 11 | 5′-AAATTCAAAAG-3′ |
| sch9Δ | 9 | Base substitution | C → A | No change | 1,347 | 5′-TTTTCGAATGG-3′ |
| sch9Δ | 10 | Insertion | T | Frameshift (T4-T5) | 1,022 | 5′-GGACTTTTTAGT-3′ |
| sch9Δ | 10 | Insertion | T | Frameshift (T6-T7) | 1,386 | 5′-CTTTTTTTGCAT-3′ |
| rev1Δ | 1 | Base substitution | C → T | Arginine-serine | 1,195 | 5′-GGTTCCCGTAT-3′ |
| rev1Δ | 2 | Insertion | A | Frameshift (A3-A4) | 702 | 5′-CAAAAGTTTA-3′ |
| rev1Δ | 3 | Deletion | G | Frameshift | 422 | 5′-GGGTGAAATGG-3′ |
| rev1Δ | 3 | Deletion | AAA | A3-A0 | 425-427 | 5′-GGGTGAAATGG-3′ |
| rev1Δ | 4 | Deletion | G | Frameshift | 422 | 5′-GGGTGAAATGG-3′ |
| rev1Δ | 4 | Deletion | AAA | A3-A0 | 425-427 | 5′-GGGTGAAATGG-3′ |
| rev1Δ | 5 | No PCR | NA | NA | NA | NA |
| rev1Δ | 6 | Base substitution | G → A | Tryptophan-STOP | 522 | 5′-GTATTGGTTTTC-3′ |
| rev1Δ | 7 | Base substitution | C → T | Serine-leucine | 1,214 | 5′-TATCAAAGAAC-3′ |
| rev1Δ | 8 | Base substitution | C → T | Histidine-tyrosine | 274 | 5′-CAAAGACATATT-3′ |
Letters in bold indicate base substitutions; bold and italics indicate insertions; underline indicates deletions. NA, not applicable.
from Madia et al. (2008).