Binding characteristics of Evasin-1 and -3 as determined by SPR
| CKBP . | Chemokine . | ka × 106 . | kd × 10−3 . | Kd × 10−9 . |
|---|---|---|---|---|
. | . | M−1s−1 . | s−1 . | nM . |
| Evasin-1 | CCL3 | 13.8 ± 3.54 | 2.43 ± 0.61 | 0.12 |
| CCL3L1 | 13.2 ± 2.9 | 0.67 ± 0.13 | 0.05 | |
| mMIP-1α | 15.6 ± 16 | 1.18 ± 1.07 | 0.08 | |
| CCL4 | 4.29 ± 2.58 | 2.2 ± 0.18 | 0.51 | |
| mMIP-1β | 3.84 ± 1.42 | 0.58 ± 0.12 | 0.15 | |
| Evasin-3 (HEK) | CXCL1 | 3.95 ± 4.23 | 1.42 ± 0.36 | 0.34 |
| CXCL8 | 0.41 ± 0.30 | 0.29 ± 0.14 | 0.7 | |
| KC | 0.64 ± 0.58 | 1.44 ± 0.35 | 2.25 | |
| MIP-2 | 0.79 ± 0.9 | 0.33 ± 0.11 | 0.4 | |
| Evasin-3 (E. coli) | CXCL1 | 2.61 ± 0.69 | 2.22 ± 0.49 | 0.85 |
| CXCL8 | 0.84 ± 0.33 | 0.36 ± 0.05 | 0.43 | |
| KC | 0.32 ± 0.17 | 1.85 ± 0.43 | 5.78 | |
| MIP-2 | 1.02 ± 0.56 | 0.65 ± 0.25 | 0.64 |
| CKBP . | Chemokine . | ka × 106 . | kd × 10−3 . | Kd × 10−9 . |
|---|---|---|---|---|
. | . | M−1s−1 . | s−1 . | nM . |
| Evasin-1 | CCL3 | 13.8 ± 3.54 | 2.43 ± 0.61 | 0.12 |
| CCL3L1 | 13.2 ± 2.9 | 0.67 ± 0.13 | 0.05 | |
| mMIP-1α | 15.6 ± 16 | 1.18 ± 1.07 | 0.08 | |
| CCL4 | 4.29 ± 2.58 | 2.2 ± 0.18 | 0.51 | |
| mMIP-1β | 3.84 ± 1.42 | 0.58 ± 0.12 | 0.15 | |
| Evasin-3 (HEK) | CXCL1 | 3.95 ± 4.23 | 1.42 ± 0.36 | 0.34 |
| CXCL8 | 0.41 ± 0.30 | 0.29 ± 0.14 | 0.7 | |
| KC | 0.64 ± 0.58 | 1.44 ± 0.35 | 2.25 | |
| MIP-2 | 0.79 ± 0.9 | 0.33 ± 0.11 | 0.4 | |
| Evasin-3 (E. coli) | CXCL1 | 2.61 ± 0.69 | 2.22 ± 0.49 | 0.85 |
| CXCL8 | 0.84 ± 0.33 | 0.36 ± 0.05 | 0.43 | |
| KC | 0.32 ± 0.17 | 1.85 ± 0.43 | 5.78 | |
| MIP-2 | 1.02 ± 0.56 | 0.65 ± 0.25 | 0.64 |