IgH SHM analysis of spleen B cells of HIS (BALB-nude-Rag/γ) mice
| B cells . | Total unique sequences . | Mutated sequencesa . | % of mutation frequencyb . | Mutation range . |
|---|---|---|---|---|
| Donor 1 IgD+CD27+ | 32 | 7 (22%) | 2.6 | 3–14 |
| Donor 2 IgD+CD27+ | 34 | 9 (26%) | 2.4 | 3–10 |
| B cells . | Total unique sequences . | Mutated sequencesa . | % of mutation frequencyb . | Mutation range . |
|---|---|---|---|---|
| Donor 1 IgD+CD27+ | 32 | 7 (22%) | 2.6 | 3–14 |
| Donor 2 IgD+CD27+ | 34 | 9 (26%) | 2.4 | 3–10 |
Only unique rearrangements were considered for analysis. Sequences scored as mutated contained three or more mutations (≥1% mutation frequency). In brackets is the frequency of mutated sequences among all sequences.
The percentage of point mutations among the total number of sequenced nucleotides of mutated sequences.